Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.11.1.21 extracted from

  • Eady, N.A.; Jesmin, N.A.; Servos, S.; Cass, A.E.; Nagy, J.M.; Brown, K.A.
    Probing the function of Mycobacterium tuberculosis catalase-peroxidase by site-directed mutagenesis (2005), Dalton Trans., 2005, 3495-3500.
    View publication on PubMed

Application

Application Comment Organism
medicine the enzyme activates the pro-antitubercular drug isoniazid Mycobacterium tuberculosis

Protein Variants

Protein Variants Comment Organism
H108L the mutant shows decreased catalase catalytic efficiency compared to the wild type enzyme Mycobacterium tuberculosis
W107F the mutant shows decreased catalase catalytic efficiency compared to the wild type enzyme Mycobacterium tuberculosis
W107F/W321F the mutant shows decreased catalase catalytic efficiency compared to the wild type enzyme Mycobacterium tuberculosis
W321F the mutant shows decreased catalase catalytic efficiency compared to the wild type enzyme Mycobacterium tuberculosis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.11
-
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) peroxidatic activity, wild type enzyme, in 10 mM KH2PO4/K2HPO4, pH 6.0, at 25°C Mycobacterium tuberculosis
0.32
-
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) peroxidatic activity, mutant enzyme W107F/W321F, in 10 mM KH2PO4/K2HPO4, pH 6.0, at 25°C Mycobacterium tuberculosis
0.36
-
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) peroxidatic activity, mutant enzyme W321F, in 10 mM KH2PO4/K2HPO4, pH 6.0, at 25°C Mycobacterium tuberculosis
0.47
-
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) peroxidatic activity, mutant enzyme W107F, in 10 mM KH2PO4/K2HPO4, pH 6.0, at 25°C Mycobacterium tuberculosis
1.6
-
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) peroxidatic activity, mutant enzyme H108L, in 10 mM KH2PO4/K2HPO4, pH 6.0, at 25°C Mycobacterium tuberculosis
5.7
-
tert-butyl hydroperoxide peroxidatic activity, mutant enzyme W107F, in 10 mM KH2PO4/K2HPO4, pH 6.0, at 25°C Mycobacterium tuberculosis
12.7
-
tert-butyl hydroperoxide peroxidatic activity, mutant enzyme H108L, in 10 mM KH2PO4/K2HPO4, pH 6.0, at 25°C Mycobacterium tuberculosis
15.5
-
tert-butyl hydroperoxide peroxidatic activity, mutant enzyme W107F/W321F, in 10 mM KH2PO4/K2HPO4, pH 6.0, at 25°C Mycobacterium tuberculosis
31.5
-
H2O2 catalatic activity, mutant enzyme W107F, in 10 mM KH2PO4/K2HPO4, pH 6.0, at 25°C Mycobacterium tuberculosis
32.1
-
H2O2 catalatic activity, mutant enzyme W321F, in 10 mM KH2PO4/K2HPO4, pH 6.0, at 25°C Mycobacterium tuberculosis
33
-
H2O2 catalatic activity, mutant enzyme W107F/W321F, in 10 mM KH2PO4/K2HPO4, pH 6.0, at 25°C Mycobacterium tuberculosis
42.6
-
H2O2 catalatic activity, wild type enzyme, in 10 mM KH2PO4/K2HPO4, pH 6.0, at 25°C Mycobacterium tuberculosis
54.9
-
H2O2 catalatic activity, mutant enzyme H108L, in 10 mM KH2PO4/K2HPO4, pH 6.0, at 25°C Mycobacterium tuberculosis
91.2
-
tert-butyl hydroperoxide peroxidatic activity, mutant enzyme W321F, in 10 mM KH2PO4/K2HPO4, pH 6.0, at 25°C Mycobacterium tuberculosis
97.9
-
tert-butyl hydroperoxide peroxidatic activity, wild type enzyme, in 10 mM KH2PO4/K2HPO4, pH 6.0, at 25°C Mycobacterium tuberculosis

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + tert-butyl hydroperoxide
-
Mycobacterium tuberculosis oxidized 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + ?
-
?
H2O2
-
Mycobacterium tuberculosis O2 + H2O
-
?
o-dianisidine + H2O2
-
Mycobacterium tuberculosis oxidized o-dianisidine + H2O
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.34
-
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) peroxidatic activity, mutant enzyme H108L, in 10 mM KH2PO4/K2HPO4, pH 6.0, at 25°C Mycobacterium tuberculosis
0.37
-
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) peroxidatic activity, mutant enzyme W321F, in 10 mM KH2PO4/K2HPO4, pH 6.0, at 25°C Mycobacterium tuberculosis
0.72
-
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) peroxidatic activity, wild type enzyme, in 10 mM KH2PO4/K2HPO4, pH 6.0, at 25°C Mycobacterium tuberculosis
2.4
-
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) peroxidatic activity, mutant enzyme W107F, in 10 mM KH2PO4/K2HPO4, pH 6.0, at 25°C Mycobacterium tuberculosis
4.8
-
H2O2 catalatic activity, mutant enzyme W107F, in 10 mM KH2PO4/K2HPO4, pH 6.0, at 25°C Mycobacterium tuberculosis
5.4
-
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) peroxidatic activity, mutant enzyme W107F/W321F, in 10 mM KH2PO4/K2HPO4, pH 6.0, at 25°C Mycobacterium tuberculosis
7.7
-
H2O2 catalatic activity, mutant enzyme W107F/W321F, in 10 mM KH2PO4/K2HPO4, pH 6.0, at 25°C Mycobacterium tuberculosis
12
-
H2O2 catalatic activity, mutant enzyme H108L, in 10 mM KH2PO4/K2HPO4, pH 6.0, at 25°C Mycobacterium tuberculosis
2300
-
H2O2 catalatic activity, mutant enzyme W321F, in 10 mM KH2PO4/K2HPO4, pH 6.0, at 25°C Mycobacterium tuberculosis
6800
-
H2O2 catalatic activity, wild type enzyme, in 10 mM KH2PO4/K2HPO4, pH 6.0, at 25°C Mycobacterium tuberculosis

Cofactor

Cofactor Comment Organism Structure
heme heme-dependent enzyme Mycobacterium tuberculosis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.014
-
tert-butyl hydroperoxide peroxidatic activity, mutant enzyme W321F, in 10 mM KH2PO4/K2HPO4, pH 6.0, at 25°C Mycobacterium tuberculosis
0.028
-
tert-butyl hydroperoxide peroxidatic activity, mutant enzyme H108L, in 10 mM KH2PO4/K2HPO4, pH 6.0, at 25°C Mycobacterium tuberculosis
0.029
-
tert-butyl hydroperoxide peroxidatic activity, wild type enzyme, in 10 mM KH2PO4/K2HPO4, pH 6.0, at 25°C Mycobacterium tuberculosis
0.15
-
H2O2 catalatic activity, mutant enzyme W107F, in 10 mM KH2PO4/K2HPO4, pH 6.0, at 25°C Mycobacterium tuberculosis
0.21
-
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) peroxidatic activity, mutant enzyme H108L, in 10 mM KH2PO4/K2HPO4, pH 6.0, at 25°C Mycobacterium tuberculosis
0.22
-
H2O2 catalatic activity, mutant enzyme H108L, in 10 mM KH2PO4/K2HPO4, pH 6.0, at 25°C Mycobacterium tuberculosis
0.23
-
H2O2 catalatic activity, mutant enzyme W107F/W321F, in 10 mM KH2PO4/K2HPO4, pH 6.0, at 25°C Mycobacterium tuberculosis
0.42
-
tert-butyl hydroperoxide peroxidatic activity, mutant enzyme W107F, in 10 mM KH2PO4/K2HPO4, pH 6.0, at 25°C Mycobacterium tuberculosis
0.48
-
tert-butyl hydroperoxide peroxidatic activity, mutant enzyme W107F/W321F, in 10 mM KH2PO4/K2HPO4, pH 6.0, at 25°C Mycobacterium tuberculosis
1
-
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) peroxidatic activity, mutant enzyme W321F, in 10 mM KH2PO4/K2HPO4, pH 6.0, at 25°C Mycobacterium tuberculosis
5.1
-
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) peroxidatic activity, mutant enzyme W107F, in 10 mM KH2PO4/K2HPO4, pH 6.0, at 25°C Mycobacterium tuberculosis
6.3
-
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) peroxidatic activity, wild type enzyme, in 10 mM KH2PO4/K2HPO4, pH 6.0, at 25°C Mycobacterium tuberculosis
17
-
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) peroxidatic activity, mutant enzyme W107F/W321F, in 10 mM KH2PO4/K2HPO4, pH 6.0, at 25°C Mycobacterium tuberculosis
73
-
H2O2 catalatic activity, mutant enzyme W321F, in 10 mM KH2PO4/K2HPO4, pH 6.0, at 25°C Mycobacterium tuberculosis
160
-
H2O2 catalatic activity, wild type enzyme, in 10 mM KH2PO4/K2HPO4, pH 6.0, at 25°C Mycobacterium tuberculosis