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Literature summary for 1.11.1.21 extracted from
- Integral role of the I-helix in the function of the "inactive" C-terminal domain of catalase-peroxidase (KatG) (2013), Biochim. Biophys. Acta, 1834, 362-371.
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| E695A | only subtle variations in spectroscopic and catalytic properties of the enzyme, substantial decrease in the rate and extent of KatG N-terminal domain reactivation | Mycobacterium tuberculosis |
| E695A/Y697A | substantial increase in hexa-coordinate low-spin heme and diminished enzyme activity, complete loss of the capacity for the reactivation of the N-terminal domain | Mycobacterium tuberculosis |
| L690A/R691A | substantial increase in hexa-coordinate low-spin heme and diminished enzyme activity, complete loss of the capacity for the reactivation of the N-terminal domain | Mycobacterium tuberculosis |
| L690A/R691A/E695A/Y697A | substantial increase in hexa-coordinate low-spin heme and diminished enzyme activity, complete loss of the capacity for the reactivation of the N-terminal domain | Mycobacterium tuberculosis |
| R691A | only subtle variations in spectroscopic and catalytic properties of the enzyme, substantial decrease in the rate and extent of KatG N-terminal domain reactivation | Mycobacterium tuberculosis |
| Y697A | only subtle variations in spectroscopic and catalytic properties of the enzyme, substantial decrease in the rate and extent of KatG N-terminal domain reactivation | Mycobacterium tuberculosis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.2 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | mutant E695A/Y697A, pH 7.0, 23°C | Mycobacterium tuberculosis |  |
| 1.9 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | mutant Y697A, pH 7.0, 23°C | Mycobacterium tuberculosis | |
| 2.2 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | mutant L690A/R691A/E695A/Y697A, pH 7.0, 23°C | Mycobacterium tuberculosis | |
| 2.4 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | wild-type, N- and C-terminal domain separately expressed, pH 7.0, 23°C | Mycobacterium tuberculosis | |
| 2.7 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | mutant L690A/R691A, pH 7.0, 23°C | Mycobacterium tuberculosis | |
| 2.7 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | mutant R691A, pH 7.0, 23°C | Mycobacterium tuberculosis | |
| 2.9 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | mutant E695A, pH 7.0, 23°C | Mycobacterium tuberculosis | |
| 3.3 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | mutant L690A/R691A, N- and C-terminal domain separately expressed, pH 7.0, 23°C | Mycobacterium tuberculosis | |
| 3.6 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | mutant E695A, N- and C-terminal domain separately expressed, pH 7.0, 23°C | Mycobacterium tuberculosis | |
| 4 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | mutant R691A, N- and C-terminal domain separately expressed, pH 7.0, 23°C | Mycobacterium tuberculosis | |
| 4.5 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | wild-type, pH 7.0, 23°C | Mycobacterium tuberculosis | |
| 6 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | mutant Y697A, N- and C-terminal domain separately expressed, pH 7.0, 23°C | Mycobacterium tuberculosis | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mycobacterium tuberculosis | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O2 | - |
Mycobacterium tuberculosis | ? + H2O | - |
? | |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 160 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | mutant L690A/R691A/E695A/Y697A, pH 7.0, 23°C | Mycobacterium tuberculosis | |
| 1020 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | mutant E695A/Y697A, pH 7.0, 23°C | Mycobacterium tuberculosis | |
| 1050 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | mutant Y697A, N- and C-terminal domain separately expressed, pH 7.0, 23°C | Mycobacterium tuberculosis | |
| 2200 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | mutant E695A, N- and C-terminal domain separately expressed, pH 7.0, 23°C | Mycobacterium tuberculosis | |
| 2700 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | mutant L690A/R691A, N- and C-terminal domain separately expressed, pH 7.0, 23°C | Mycobacterium tuberculosis | |
| 3500 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | mutant R691A, N- and C-terminal domain separately expressed, pH 7.0, 23°C | Mycobacterium tuberculosis | |
| 4000 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | wild-type, N- and C-terminal domain separately expressed, pH 7.0, 23°C | Mycobacterium tuberculosis | |
| 4800 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | mutant L690A/R691A, pH 7.0, 23°C | Mycobacterium tuberculosis | |
| 6800 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | mutant R691A, pH 7.0, 23°C | Mycobacterium tuberculosis | |
| 8300 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | mutant Y697A, pH 7.0, 23°C | Mycobacterium tuberculosis | |
| 8900 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | mutant E695A, pH 7.0, 23°C | Mycobacterium tuberculosis | |
| 11500 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | wild-type, pH 7.0, 23°C | Mycobacterium tuberculosis | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 70 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | mutant L690A/R691A/E695A/Y697A, pH 7.0, 23°C | Mycobacterium tuberculosis | |
| 200 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | mutant Y697A, N- and C-terminal domain separately expressed, pH 7.0, 23°C | Mycobacterium tuberculosis | |
| 600 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | mutant E695A, N- and C-terminal domain separately expressed, pH 7.0, 23°C | Mycobacterium tuberculosis | |
| 800 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | mutant L690A/R691A, N- and C-terminal domain separately expressed, pH 7.0, 23°C | Mycobacterium tuberculosis | |
| 850 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | mutant E695A/Y697A, pH 7.0, 23°C | Mycobacterium tuberculosis | |
| 900 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | mutant R691A, N- and C-terminal domain separately expressed, pH 7.0, 23°C | Mycobacterium tuberculosis | |
| 1700 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | mutant L690A/R691A, pH 7.0, 23°C | Mycobacterium tuberculosis | |
| 1700 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | wild-type, N- and C-terminal domain separately expressed, pH 7.0, 23°C | Mycobacterium tuberculosis | |
| 2500 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | mutant R691A, pH 7.0, 23°C | Mycobacterium tuberculosis | |
| 2500 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | wild-type, pH 7.0, 23°C | Mycobacterium tuberculosis | |
| 3100 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | mutant E695A, pH 7.0, 23°C | Mycobacterium tuberculosis | |
| 4400 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | mutant Y697A, pH 7.0, 23°C | Mycobacterium tuberculosis | |
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