Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) Star cells | Pyricularia grisea |
Crystallization (Comment) | Organism |
---|---|
sitting drop vapor diffusion method, using 15% (w/v) PEG 4000 and 0.1 M sodium acetate (pH 4.6) | Pyricularia grisea |
Protein Variants | Comment | Organism |
---|---|---|
R461A | the mutation slightly increases the thermal stability but does not alter the active site architecture or the kinetics of cyanide binding. However, the variant loses the wild type-typical optimum of catalase activity at pH 5.3 but exhibits a broad plateau between pH 4.5 and 7.5 | Pyricularia grisea |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyricularia grisea | A4QUT2 | - |
- |
Pyricularia grisea 70-15 | A4QUT2 | - |
- |
Purification (Comment) | Organism |
---|---|
chelating Sepharose column chromatography, and hydroxyapatite column chromatography | Pyricularia grisea |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 H2O2 | - |
Pyricularia grisea | O2 + H2O | - |
? | |
2 H2O2 | - |
Pyricularia grisea 70-15 | O2 + H2O | - |
? | |
guaiacol + H2O2 | - |
Pyricularia grisea | tetraguaiacol + H2O | - |
? | |
guaiacol + H2O2 | - |
Pyricularia grisea 70-15 | tetraguaiacol + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
KatG2 | - |
Pyricularia grisea |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
53 | - |
melting temperatures are at 53 and 63°C | Pyricularia grisea |
63 | - |
melting temperatures are at 53 and 63°C | Pyricularia grisea |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
270 | - |
H2O2 | mutant enzyme R461A, at pH 5.5 and 30°C | Pyricularia grisea | |
6450 | - |
H2O2 | wild type enzyme, at pH 5.3 and 30°C | Pyricularia grisea |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.3 | - |
- |
Pyricularia grisea |