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Literature summary for 1.11.1.21 extracted from
- Peculiar genes for thermostable bifunctional catalase-peroxidases in Chaetomium thermophilum and their molecular evolution (2018), Gene, 666, 83-91 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| fungal gene katG, without introns, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis. The CthediskatG gene is in the genome embedded by normal eukaryotic transcriptional regulation elements within 5' and 3'-UTR. Quantitative reverse-transcription real-time PCR enzyme expression analysis | Thermochaetoides dissita |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | in the heme group | Thermochaetoides dissita |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermochaetoides dissita | A0A223PYP9 | - |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| additional information | best growth of the fungal culture on agar plates at 45-50°C, highest enzyme expression level from CthediskatG gene at 55°C | Thermochaetoides dissita | - |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CthediskatG | - |
Thermochaetoides dissita |
| KatG | - |
Thermochaetoides dissita |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| heme | - |
Thermochaetoides dissita | |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Thermochaetoides dissita | the enzyme is induced by H2O2, best at 55°C | up |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | catalase-peroxidases represent one important subfamily of ancestral antioxidant enzymes originally evolved in bacteria for the protection against various forms of oxidative stress. KatG genes coding for these bifunctional catalase-peroxidases were during their peculiar evolution transferred from Bacteroidetes to the fungal phylum Ascomycota via a horizontal gene transfer event. Identification of the gene for thermostable bifunctional catalase-peroxidases in Chaetomium thermophilum and their molecular evolution, overview. The gene from Chaetomium thermophilum, CthediskatG, resembling its bacterial counterparts has a typical eukaryotic transcription start site and also contains a conserved eukaryotic polyadenylation signal behind its 3' terminus. PolyA tails are detected in corresponding transcripts of katG from two different mRNA libraries of Chaetomium thermophilum thermophilum var. disstum. Although otherwise highly conserved, a unique 60 bp long deletion leading in the translated product with high probability to a modified loop and thus access to the prosthetic heme group is observed in katG genes of only two Chaetomium thermophilum variants. Molecular phylogeny revealing the evolutionary position of fungal thermostable catalase-peroxidases within a robust phylogenetic tree of the whole KatG subfamily, overview. Molecular phylogeny and phylogenetic tree | Thermochaetoides dissita |
| malfunction | detection of a unique deletion in CthediskatG gene leads to a shortened large loop 1 that can have an impact on a modified accessibility to the heme active site from the protein surface and overall stability of this enzyme | Thermochaetoides dissita |
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Release 2023.1 (January 2023)
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