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Literature summary for 1.11.1.21 extracted from
- Characterization of two catalase-peroxidase-encoding genes in Fusarium verticillioides reveals differential responses to inxa0vitro versus inxa0planta oxidative challenges (2018), Mol. Plant Pathol., 19, 1127-1139 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene FvCP01, DNA and amino acid sequence determination and analysis, quantitative reverse transcription PCR analysis of gene FvCP01 expression under H2O2 exposure | Fusarium verticillioides |
| gene FvCP02, DNA and amino acid sequence determination and analysis, quantitative reverse transcription PCR transcription analysis of gene FvCP02 under H2O2 exposure | Fusarium verticillioides |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | generation of single and double gene deletion mutants of genes FvCP01 and FvCP02 encoding isozyme in KatG1 and KatG2, respectively, in the maize pathogen Fusarium verticillioides. Both mutants DELTAFvCP01 and DELTAFvCP02 are more sensitive to H2O2 than the wild-type in vitro, although their sensitivity differ depending on the type of inoculum. Inoculations using mycelial agar plugs demonstrate an additive effect of the mutants, with the DELTAFvCP01/DELTAFvCP02 double deletion being the most sensitive to H2O2. In general, conidia are much more sensitive than agar plugs to H2O2, and conidial inoculations indicate that FvCP01 confers more H2O2 tolerance than FvCP02. Phenotypes, detailed overview | Fusarium verticillioides |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | the enzyme is secreted | Fusarium verticillioides | - |
- |
| intracellular | - |
Fusarium verticillioides | 5622 | - |
| additional information | occurrence of an N-terminal signal sequence for secretion | Fusarium verticillioides | - |
- |
| peroxisome | - |
Fusarium verticillioides | 5777 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | the heme group | Fusarium verticillioides |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2 H2O2 | Fusarium verticillioides | peroxidase activity | O2 + 2 H2O | - |
? | |
| 2 H2O2 | Fusarium verticillioides M3125 | peroxidase activity | O2 + 2 H2O | - |
? | |
| 2 H2O2 | Fusarium verticillioides FGSC 7600 | peroxidase activity | O2 + 2 H2O | - |
? | |
| donor + H2O2 | Fusarium verticillioides | catalase activity | oxidized donor + 2 H2O | - |
? | |
| donor + H2O2 | Fusarium verticillioides M3125 | catalase activity | oxidized donor + 2 H2O | - |
? | |
| donor + H2O2 | Fusarium verticillioides FGSC 7600 | catalase activity | oxidized donor + 2 H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Fusarium verticillioides | W7MLS8 | Gibberella moniliformis | - |
| Fusarium verticillioides | W7NEP8 | Gibberella moniliformis | - |
| Fusarium verticillioides FGSC 7600 | W7MLS8 | Gibberella moniliformis | - |
| Fusarium verticillioides FGSC 7600 | W7NEP8 | Gibberella moniliformis | - |
| Fusarium verticillioides M3125 | W7MLS8 | Gibberella moniliformis | - |
| Fusarium verticillioides M3125 | W7NEP8 | Gibberella moniliformis | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| conidium | - |
Fusarium verticillioides | - |
| additional information | growth on seeds of the susceptible maize cultivar Silver Queen | Fusarium verticillioides | - |
| mycelium | - |
Fusarium verticillioides | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2 H2O2 | peroxidase activity | Fusarium verticillioides | O2 + 2 H2O | - |
? | |
| 2 H2O2 | peroxidase activity | Fusarium verticillioides M3125 | O2 + 2 H2O | - |
? | |
| 2 H2O2 | peroxidase activity | Fusarium verticillioides FGSC 7600 | O2 + 2 H2O | - |
? | |
| donor + H2O2 | catalase activity | Fusarium verticillioides | oxidized donor + 2 H2O | - |
? | |
| donor + H2O2 | catalase activity | Fusarium verticillioides M3125 | oxidized donor + 2 H2O | - |
? | |
| donor + H2O2 | catalase activity | Fusarium verticillioides FGSC 7600 | oxidized donor + 2 H2O | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| FvCP01 | - |
Fusarium verticillioides |
| FvCP02 | - |
Fusarium verticillioides |
| FVEG_10866 | locus name | Fusarium verticillioides |
| FVEG_12888 | locus name | Fusarium verticillioides |
| KatG1 | - |
Fusarium verticillioides |
| KatG2 | - |
Fusarium verticillioides |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| heme | - |
Fusarium verticillioides | |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Fusarium verticillioides | transcriptional analysis shows the induction of FvCP01, but decreased expression of FvCP02, in both mycelia and spores in the wild-type after H2O2 exposure, but this trend is reversed when the fungus is grown on germinating maize seeds. This interaction with the plant increases the expression of FvCP02, but not FvCP01, indicating that FvCP02 may be responsive to plant-derived H2O2. FvCP01 is induced more than threefold in the DFvCP02 mutant grown on germlings, suggesting that FvCP01 can compensate for the loss of FvCP02 | down |
| Fusarium verticillioides | transcriptional analysis shows the induction of FvCP01, but decreased expression of FvCP02, in both mycelia and spores in the wild-type after H2O2 exposure, but this trend is reversed when the fungus is grown on germinating maize seeds. This interaction with the plant increases the expression of FvCP02, but not FvCP01, indicating that FvCP02 may be responsive to plant-derived H2O2. FvCP01 is induced more than threefold in the DFvCP02 mutant grown on germlings, suggesting that FvCP01 can compensate for the loss of FvCP02 | up |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | catalase-peroxidases (KatGs) are a superfamily of reactive oxygen species (ROS)-degrading enzymes believed to have been horizontally acquired by ancient Ascomycota from bacteria. Subsequent gene duplication resulted in two KatG paralogues in ascomycetes: the widely distributed intracellular KatG1 group and the phytopathogen-dominated extracellular KatG2 group | Fusarium verticillioides |
| physiological function | differing roles of isozymes FvCP01 and FvCP02 in protective responses against H2O2-derived oxidative stress, modeling. Genes FvCP01 and FvCP02 may play a minor role in the virulence of Fusarium verticillioides | Fusarium verticillioides |
| physiological function | differing roles of isozymes FvCP01 and FvCP02 in protective responses against H2O2-derived oxidative stress, modeling. In addition, KatG2 genes, because they are mostly limited to fungal phytopathogens, have been inferred to play a potential role in host-pathogen interactions. Genes FvCP01 and FvCP02 may play a minor role in the virulence of Fusarium verticillioides | Fusarium verticillioides |
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