Literature summary for 1.11.1.24 extracted from

Castro, H.; Budde, H.; Flohe, L.; Hofmann, B.; Lunsdorf, H.; Wissing, J.; Tomas, A.M.
Specificity and kinetics of a mitochondrial peroxiredoxin of Leishmania infantum (2002), Free Radic. Biol. Med., 33, 1563-1574.

Search for more literature for 1.11.1.24

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli as an N-terminally His-tagged protein	Leishmania infantum

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0091	-	tert-butyl hydroperoxide	pH 7.6, 25°C	Leishmania infantum
0.0319	-	tryparedoxin 2	pH 7.6, 25°C	Leishmania infantum

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
mitochondrion	-	Leishmania infantum	5739	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
27345	-	10 * 27345, the enzyme exists as a homodecameric ring structure composed of five dimers, MALDI-TOF spectrometry	Leishmania infantum

Organism

Organism	UniProt	Comment	Textmining
Leishmania infantum	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
cumene hydroperoxide + tryparedoxin 2	57% of the activity with H2O2	Leishmania infantum	2-phenylpropan-2-ol + oxidized tryparedoxin 2	-	?
H2O2 + tryparedoxin 2	-	Leishmania infantum	H2O + oxidized tryparedoxin 2	-	?
linoleic acid hydroperoxide + tryparedoxin 2	7.8% of the activity with H2O2	Leishmania infantum	?	-	?
additional information	tryparedoxin peroxidase activity. The enzyme does not follow a classic ping-pong mechanism	Leishmania infantum	?	-	?
phosphatidyl choline hydroperoxide + tryparedoxin 2	3.8% of the activity with H2O2	Leishmania infantum	?	-	?
tert-butyl hydroperoxide + reduced thioredoxin	9.5% of the activity with tert-butyl hydroperoxide and tryparedoxin 2	Leishmania infantum	tert-butanol + oxidized thioredoxin + H2O	-	?
tert-butyl hydroperoxide + tryparedoxin 2	95% of the activity with H2O2	Leishmania infantum	tert-butanol + oxidized tryparedoxin 2	-	?

Subunits

Subunits	Comment	Organism
decamer	10 * 27345, the enzyme exists as a homodecameric ring structure composed of five dimers, MALDI-TOF spectrometry	Leishmania infantum

Synonyms

Synonyms	Comment	Organism
LimTXNPx	-	Leishmania infantum
peroxiredoxin	-	Leishmania infantum

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
37	-	tryparedoxin 2	pH 7.6, 25°C	Leishmania infantum

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)