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Literature summary for 1.11.1.24 extracted from
- Cloning and characterization of a 2-Cys peroxiredoxin from Pisum sativum (2004), J. Exp. Bot., 55, 2191-2199.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression using vector pET3d | Pisum sativum |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0276 | - |
H2O2 | - |
Pisum sativum |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 21913 | - |
x * 21913, calculation from nucleotide sequence | Pisum sativum |
| 49000 | - |
dimer, gel filtration | Pisum sativum |
| 388000 | - |
decamer, gel filtration | Pisum sativum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pisum sativum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pisum sativum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| H2O2 + dithiothreitol | - |
Pisum sativum | H2O + oxidized dithiothreitol | - |
? | |
| additional information | thioredoxin m is more efficent than thioredoxin f in reducing the enzyme | Pisum sativum | ? | - |
? | |
| tert-butyl hydroperoxide + dithiothreitol | - |
Pisum sativum | tert-butanol + oxidized dithiothreitol | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 21913, calculation from nucleotide sequence | Pisum sativum |
| More | the transition dimer-decamer produced in vitro between pH 7.5 and 8.0 suggests that a great change in the enzyme quarternary structure of the enzyme may take place in the chloroplast during the dark-light transition. The dimer-decamer equilibrium depends on NaCl concentration and concentration of dithiothreitol | Pisum sativum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 2-Cys peroxiredoxin | - |
Pisum sativum |
| 2-Cys Prx | - |
Pisum sativum |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.69 | - |
H2O2 | - |
Pisum sativum | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| thioredoxin | thioredoxin m is more efficent than thioredoxin f in reducing the enzyme | Pisum sativum | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Pisum sativum | calculation from nucleotide sequence | - |
4.9 |
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