Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Mycobacterium tuberculosis |
General Stability | Organism |
---|---|
addition of 1 mM N-acetylcysteine to AhpE treated with equimolar H2O2 results in a destabilization of the dimer, leading to lower molecular weight species | Mycobacterium tuberculosis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
19320 | - |
electrospray ionization mass spectrometry | Mycobacterium tuberculosis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
H2O2 + reduced thioredoxin | - |
Mycobacterium tuberculosis | H2O + oxidized thioredoxin | - |
? | |
peroxynitrite + reduced thioredoxin | AhpE reduces peroxynitrite 2 orders of magnitude faster than H2O2 | Mycobacterium tuberculosis | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AhpE | a 1-Cys peroxiredoxin | Mycobacterium tuberculosis |
alkyl hydroperoxide reductase | - |
Mycobacterium tuberculosis |
Rv2238c | - |
Mycobacterium tuberculosis |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
AhpE is unstable and precipitates under low pH conditions (below pH 4.5) | Mycobacterium tuberculosis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
thioredoxin | - |
Mycobacterium tuberculosis |