Cloned (Comment) | Organism |
---|---|
- |
Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
C45S | mutation results in a complete loss of thiol peroxidase activity | Escherichia coli |
C50S | mutant enzyme show about 55% of wild-type activity with H2O2 as substrate | Escherichia coli |
C99S | mutant enzyme show about 95% of wild-type activity with H2O2 as substrate | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.117 | - |
linoleic acid hydroperoxide | pH 7.0, 37°C | Escherichia coli | |
0.374 | - |
tert-butyl hydroperoxide | pH 7.0, 37°C | Escherichia coli | |
0.478 | - |
H2O2 | pH 7.0, 37°C | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
thioredoxin + linoleic acid hydroperoxide | Escherichia coli | the enzyme defends against oxidative stress through decomposition of hydroperoxide | thioredoxin disulfide + H2O + linoleic acid | - |
? | |
thioredoxin + linoleic acid hydroperoxide | Escherichia coli K12 | the enzyme defends against oxidative stress through decomposition of hydroperoxide | thioredoxin disulfide + H2O + linoleic acid | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P0AE52 | - |
- |
Escherichia coli K12 | P0AE52 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
thioredoxin + H2O2 | the Vmax/Km value is about 5fold lower than the Vmax/Km value linoleic acid hydroperoxide | Escherichia coli | thioredoxin disulfide + 2 H2O | - |
? | |
thioredoxin + H2O2 | the Vmax/Km value is about 5fold lower than the Vmax/Km value linoleic acid hydroperoxide | Escherichia coli K12 | thioredoxin disulfide + 2 H2O | - |
? | |
thioredoxin + linoleic acid hydroperoxide | - |
Escherichia coli | thioredoxin disulfide + H2O + linoleic acid | - |
? | |
thioredoxin + linoleic acid hydroperoxide | the enzyme defends against oxidative stress through decomposition of hydroperoxide | Escherichia coli | thioredoxin disulfide + H2O + linoleic acid | - |
? | |
thioredoxin + linoleic acid hydroperoxide | - |
Escherichia coli K12 | thioredoxin disulfide + H2O + linoleic acid | - |
? | |
thioredoxin + linoleic acid hydroperoxide | the enzyme defends against oxidative stress through decomposition of hydroperoxide | Escherichia coli K12 | thioredoxin disulfide + H2O + linoleic acid | - |
? | |
thioredoxin + tert-butyl hydroperoxide | the Vmax/Km value is about 14fold lower than the Vmax/Km value linoleic acid hydroperoxide | Escherichia coli | thioredoxin disulfide + H2O + tert-butyl alcohol | - |
? | |
thioredoxin + tert-butyl hydroperoxide | the Vmax/Km value is about 14fold lower than the Vmax/Km value linoleic acid hydroperoxide | Escherichia coli K12 | thioredoxin disulfide + H2O + tert-butyl alcohol | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | - |
Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
bacterioferritin comigratory protein | - |
Escherichia coli |
BCP | - |
Escherichia coli |
Organism | Comment | Expression |
---|---|---|
Escherichia coli | the expression level gradually elevates during exponential growth until mid-log phase growth beyond which the expression level is decreased | down |
Escherichia coli | the expression level gradually elevates during exponential growth until mid-log phase growth. The enzyme (BCP) is induced 3fold by the oxidative stress given by changing the growth conditions from the anaerobic to aerobic culture | up |
General Information | Comment | Organism |
---|---|---|
malfunction | Bcp null mutant grows more slowly than its wild type in aerobic culture and shows the hypersensitivity toward various oxidants such as H2O2, tert-butyl hydroperoxide, and linoleic acid hydroperoxide | Escherichia coli |
physiological function | the enzyme defends against oxidative stress through decomposition of hydroperoxide | Escherichia coli |