Cloned (Comment) | Organism |
---|---|
expression of V197C/C128A mutant in Escherichia coli | Saccharomyces cerevisiae |
Crystallization (Comment) | Organism |
---|---|
with comercial kit | Saccharomyces cerevisiae |
Protein Variants | Comment | Organism |
---|---|---|
V197C/C128A | as active as the wild-type enzyme. Used to generate a covalent complex with a mutant cytochrome c | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ferrocytochrome c + H2O2 | Saccharomyces cerevisiae | - |
ferricytochrome c + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
high-purity V197C/C128A mutant is obtained after an anion-exchange chromatography and gel filtration | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ferrocytochrome c + H2O2 | - |
Saccharomyces cerevisiae | ferricytochrome c + H2O | - |
? | |
horse heart ferrocytochrome c + H2O2 | - |
Saccharomyces cerevisiae | horse heart ferricytochrome c + H2O | - |
r | |
yeast ferrocytochrome c + H2O2 | - |
Saccharomyces cerevisiae | yeast ferricytochrome c + H2O | - |
r |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.2 | - |
horse heart ferrocytochrome c | pH 6, 200 mM potassium phosphate, covalent complex | Saccharomyces cerevisiae | |
15.7 | - |
yeast ferrocytochrome c | pH 6, 20 mM potassium phosphate, covalent complex | Saccharomyces cerevisiae | |
20.7 | - |
horse heart ferrocytochrome c | pH 6, 20 mM potassium phosphate, covalent complex | Saccharomyces cerevisiae | |
76.2 | - |
yeast ferrocytochrome c | pH 6, 200 mM potassium phosphate, covalent complex | Saccharomyces cerevisiae | |
166.8 | - |
horse heart ferrocytochrome c | pH 6, 200 mM potassium phosphate, V197C/C128A mutant | Saccharomyces cerevisiae | |
175.1 | - |
horse heart ferrocytochrome c | pH 6, 200 mM potassium phosphate, wild-type enzyme | Saccharomyces cerevisiae | |
219.1 | - |
yeast ferrocytochrome c | pH 6, 20 mM potassium phosphate, wild-type enzyme | Saccharomyces cerevisiae | |
240.9 | - |
yeast ferrocytochrome c | pH 6, 20 mM potassium phosphate, V197C/C128A mutant | Saccharomyces cerevisiae | |
803.4 | - |
horse heart ferrocytochrome c | pH 6, 20 mM potassium phosphate, V197C/C128A mutant | Saccharomyces cerevisiae | |
850.3 | - |
horse heart ferrocytochrome c | pH 6, 20 mM potassium phosphate, wild-type enzyme | Saccharomyces cerevisiae | |
1167 | - |
yeast ferrocytochrome c | pH 6, 200 mM potassium phosphate, V197C/C128A mutant | Saccharomyces cerevisiae | |
1362 | - |
yeast ferrocytochrome c | pH 6, 200 mM potassium phosphate, wild-type enzyme | Saccharomyces cerevisiae |