General Stability | Organism |
---|---|
free and immobilized catalases show their maximum activities at 50 mM buffer concentration. When the buffer concentration is increased from 25 to 100 mM, the activity of Eupergit C-immobilized catalase is more affected than the activity of free catalase. At 100 mM buffer concentration, free catalase retains 96.9% of its maximum activity although immobilized catalase retains 80% of its maximum activity | Bos taurus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
28.6 | - |
H2O2 | free catalase, in 50 mM phosphate buffer pH 7.0, at 25°C | Bos taurus | |
95.9 | - |
H2O2 | catalase covalently immobilized onto Eupergit C, in 50 mM phosphate buffer pH 7.0, at 25°C | Bos taurus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Bos taurus | - |
Storage Stability | Organism |
---|---|
22°C and 5°C, free enzyme, 11 days, 32% loss of activity | Bos taurus |
22°C, Eupergit C-immobilized enzyme, 11 days, 21% loss of activity | Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
H2O2 | - |
Bos taurus | O2 + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CAT | - |
Bos taurus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
free enzyme | Bos taurus |
40 | - |
Eupergit C-immobilized catalase | Bos taurus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 50 | the half lives of free catalase at 40 and 50°C are 9.0 and 6.7 h, respectively, and correspondingly 29.1 and 4.8 h for Eupergit C-immobilized catalase | Bos taurus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
Eupergit C-immobilized enzyme | Bos taurus |
7.5 | - |
free enzyme | Bos taurus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 7.5 | the activity of Eupergit C-immobilized catalase remains almost constant for pH values 6.0-7.5 whereas this is not case for the free catalase | Bos taurus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | - |
Bos taurus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Bos taurus | isoelectric focusing | - |
5.4 |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
160 | - |
H2O2 | catalase covalently immobilized onto Eupergit C, in 50 mM phosphate buffer pH 7.0, at 25°C | Bos taurus | |
21000 | - |
H2O2 | free catalase, in 50 mM phosphate buffer pH 7.0, at 25°C | Bos taurus |