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Literature summary for 1.11.1.6 extracted from

  • Alptekin, O.; Tuekel, S.; Yildirim, D.; Alagoez, D.
    Immobilization of catalase onto Eupergit C and its characterization (2010), J. Mol. Catal. B, 64, 177-183.
No PubMed abstract available

General Stability

General Stability Organism
free and immobilized catalases show their maximum activities at 50 mM buffer concentration. When the buffer concentration is increased from 25 to 100 mM, the activity of Eupergit C-immobilized catalase is more affected than the activity of free catalase. At 100 mM buffer concentration, free catalase retains 96.9% of its maximum activity although immobilized catalase retains 80% of its maximum activity Bos taurus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
28.6
-
H2O2 free catalase, in 50 mM phosphate buffer pH 7.0, at 25°C Bos taurus
95.9
-
H2O2 catalase covalently immobilized onto Eupergit C, in 50 mM phosphate buffer pH 7.0, at 25°C Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Bos taurus
-

Storage Stability

Storage Stability Organism
22°C and 5°C, free enzyme, 11 days, 32% loss of activity Bos taurus
22°C, Eupergit C-immobilized enzyme, 11 days, 21% loss of activity Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
H2O2
-
Bos taurus O2 + H2O
-
?

Synonyms

Synonyms Comment Organism
CAT
-
Bos taurus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
free enzyme Bos taurus
40
-
Eupergit C-immobilized catalase Bos taurus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40 50 the half lives of free catalase at 40 and 50°C are 9.0 and 6.7 h, respectively, and correspondingly 29.1 and 4.8 h for Eupergit C-immobilized catalase Bos taurus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
Eupergit C-immobilized enzyme Bos taurus
7.5
-
free enzyme Bos taurus

pH Range

pH Minimum pH Maximum Comment Organism
6 7.5 the activity of Eupergit C-immobilized catalase remains almost constant for pH values 6.0-7.5 whereas this is not case for the free catalase Bos taurus

Cofactor

Cofactor Comment Organism Structure
heme
-
Bos taurus

pI Value

Organism Comment pI Value Maximum pI Value
Bos taurus isoelectric focusing
-
5.4

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
160
-
H2O2 catalase covalently immobilized onto Eupergit C, in 50 mM phosphate buffer pH 7.0, at 25°C Bos taurus
21000
-
H2O2 free catalase, in 50 mM phosphate buffer pH 7.0, at 25°C Bos taurus