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Literature summary for 1.11.1.6 extracted from

  • Yuzugullu, Y.; Trinh, C.H.; Fairhurst, L.; Ogel, Z.B.; McPherson, M.J.; Pearson, A.R.
    Investigating the active centre of the Scytalidium thermophilum catalase (2013), Acta Crystallogr. Sect. D, 69, 369-375.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type and mutant enzymes in Escherichia coli strain BL21 Star (DE3) Mycothermus thermophilus

Crystallization (Commentary)

Crystallization (Comment) Organism
native and recombinant wild-type enzyme and enzyme mutants H82N and V123F, sitting drop vapour diffusion method, mixing of 9.4 mg/ml protein with reservoir solution containing 18% w/v PEG 2000, 0.1 M barium chloride, 0.1 M bis-tris pH 6.8 for the wild-type enzyme, and 6-16% w/v PEG 400, 0.2 M potassium chloride, 0.01 M calcium chloride, 0.05 M sodium cacodylate, pH 5.0-5.6 for the mutant H82N or PEG 4000 instead of PEG 400 for the mutant V123F, X-ray diffraction structure determination and analysis at 2.7 A, 1.4 A, 1.5 A, and 1.9 A resolution, respectively Mycothermus thermophilus

Protein Variants

Protein Variants Comment Organism
H82N site-directed mutagenesis, the mutation results in conversion of the native d-type heme to a b-type heme Mycothermus thermophilus
V123F site-directed mutagenesis Mycothermus thermophilus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
79000
-
4 * 79000, wild-type and mutant enzymes, SDS-PAGE Mycothermus thermophilus
320000
-
-
Mycothermus thermophilus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2 H2O2 Mycothermus thermophilus
-
O2 + 2 H2O
-
?
2 H2O2 Mycothermus thermophilus ATCC 16454
-
O2 + 2 H2O
-
?
additional information Mycothermus thermophilus the bifunctional catalase with phenol oxidase activity catalyses the decomposition of hydrogen peroxide into oxygen and water and also oxidizes various phenolic compounds ?
-
?
additional information Mycothermus thermophilus ATCC 16454 the bifunctional catalase with phenol oxidase activity catalyses the decomposition of hydrogen peroxide into oxygen and water and also oxidizes various phenolic compounds ?
-
?

Organism

Organism UniProt Comment Textmining
Mycothermus thermophilus
-
gene catpo
-
Mycothermus thermophilus ATCC 16454
-
gene catpo
-

Purification (Commentary)

Purification (Comment) Organism
recombinant wild-type and mutant enzymes from Escherichia coli strain BL21 Star (DE3) to homogeneity by anion exchange chromatography and gel filtration Mycothermus thermophilus

Reaction

Reaction Comment Organism Reaction ID
2 H2O2 = O2 + 2 H2O reaction mechanism, overview Mycothermus thermophilus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4
-
mutant H82N, pH 7.0, 60°C Mycothermus thermophilus
65
-
mutant V123F, pH 7.0, 60°C Mycothermus thermophilus
8870
-
native wild-type enzyme, pH 7.0, 60°C Mycothermus thermophilus
18710
-
recombinant wild-type enzyme, pH 7.0, 60°C Mycothermus thermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 H2O2
-
Mycothermus thermophilus O2 + 2 H2O
-
?
2 H2O2 via Compound I and Compound II intermediates Mycothermus thermophilus O2 + 2 H2O
-
?
2 H2O2
-
Mycothermus thermophilus ATCC 16454 O2 + 2 H2O
-
?
2 H2O2 via Compound I and Compound II intermediates Mycothermus thermophilus ATCC 16454 O2 + 2 H2O
-
?
additional information the bifunctional catalase with phenol oxidase activity catalyses the decomposition of hydrogen peroxide into oxygen and water and also oxidizes various phenolic compounds Mycothermus thermophilus ?
-
?
additional information the bifunctional catalase with phenol oxidase activity catalyses the decomposition of hydrogen peroxide into oxygen and water and also oxidizes various phenolic compounds Mycothermus thermophilus ATCC 16454 ?
-
?

Subunits

Subunits Comment Organism
homotetramer 4 * 79000, wild-type and mutant enzymes, SDS-PAGE Mycothermus thermophilus

Synonyms

Synonyms Comment Organism
CATPO
-
Mycothermus thermophilus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
assay at Mycothermus thermophilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
assay at Mycothermus thermophilus

Cofactor

Cofactor Comment Organism Structure
additional information the heme component is cis-hydroxychlorin gamma-spirolactone Mycothermus thermophilus

General Information

General Information Comment Organism
additional information the heme-binding pocket contains two highly conserved water molecules on the distal side Mycothermus thermophilus