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Literature summary for 1.11.1.7 extracted from
- Purification and partial characterization of broccoli (Brassica oleracea var. Italica) peroxidases (2005), J. Agric. Food Chem., 53, 3206-3214.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.042 | - |
H2O2 | 25°C, pH 6.0 | Brassica oleracea |  |
| 0.13 | - |
H2O2 | 25°C, pH 6.0 | Brassica oleracea | |
| 0.3 | - |
guaiacol | 25°C, pH 6.0 | Brassica oleracea | |
| 0.7 | - |
guaiacol | 25°C, pH 6.0 | Brassica oleracea | |
| 8.8 | - |
guaiacol | 25°C, pH 6.0 | Brassica oleracea | |
| 9.7 | - |
H2O2 | 25°C, pH 6.0 | Brassica oleracea | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| soluble | - |
Brassica oleracea | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 43000 | - |
x * 43000, SDS-PAGE | Brassica oleracea |
| 48000 | - |
x * 48000, SDS-PAGE | Brassica oleracea |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Brassica oleracea | - |
var. italica | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | - |
Brassica oleracea |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate, DEAE-Sephacel, Sephadex G-100 | Brassica oleracea |
| ammonium sulfate, DEAE-Sephacel, Sephadex G-100, Phenyl-Sepharose | Brassica oleracea |
| ammonium sulfate, DEAE-Sephacel, SP-Sepharose, Sephadex G-100, Phenyl-Sepharose | Brassica oleracea |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| stem | - |
Brassica oleracea | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 156 | - |
basic peroxidase | Brassica oleracea |
| 472 | - |
neutral peroxidase | Brassica oleracea |
| 882 | - |
acidic peroxidase | Brassica oleracea |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| guaiacol + H2O2 | - |
Brassica oleracea | tetraguaiacol + H2O | - |
? | |
| guaiacol + H2O2 | - |
Brassica oleracea | ? + H2O | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 48000, SDS-PAGE | Brassica oleracea |
| ? | x * 43000, SDS-PAGE | Brassica oleracea |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| acidic peroxidase | - |
Brassica oleracea |
| acidic POD | - |
Brassica oleracea |
| basic peroxidase | - |
Brassica oleracea |
| basic POD | - |
Brassica oleracea |
| neutral peroxidase | - |
Brassica oleracea |
| neutral POD | - |
Brassica oleracea |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 5 | - |
Brassica oleracea |
| 6 | - |
- |
Brassica oleracea |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 9 | almost no activity at pH 3.0 and pH 9.0, respectively | Brassica oleracea |
| 3 | 9 | almost no activity at pH 3.0, approx. 20% of maximal activity at pH 9.0 | Brassica oleracea |
| 3 | 9 | approx. 20% of maximal activity at pH 3.0 and pH 8.0, respectively | Brassica oleracea |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Brassica oleracea | higher than 4.0, isoelectric focusing | - |
additional information |
| Brassica oleracea | isoelectric focusing | - |
5 |
| Brassica oleracea | isoelectric focusing | - |
8 |
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