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Literature summary for 1.11.1.7 extracted from
- Structural and kinetic properties of a novel purple acid phosphatase from phosphate-starved tomato (Lycopersicon esculentum) cell cultures (2004), Biochem. J., 377, 419-428.
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| intracellular | - |
Solanum lycopersicum | 5622 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | the peroxidase activity of the enzyme is insensitive to Mg2+, Zn2+, phosphate, and molydan | Solanum lycopersicum |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 57000 | - |
1 * 63000, alpha-subunit, + 1 * 57000, beta-subunit, SDS-PAGE | Solanum lycopersicum |
| 63000 | - |
1 * 63000, alpha-subunit, + 1 * 57000, beta-subunit, SDS-PAGE | Solanum lycopersicum |
| 142000 | - |
native PAGE | Solanum lycopersicum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Solanum lycopersicum | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | - |
Solanum lycopersicum |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| intracellular enzyme 476fold to homogeneity from phosphate-starved cell culture by PEG 8000 treatment, adsorption chromatography, gel filtration, and concanavalin A affinity chromatography | Solanum lycopersicum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell culture | phosphate-starved | Solanum lycopersicum | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 10 | - |
purified enzyme | Solanum lycopersicum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5-aminophthalhydrazide + H2O2 | i.e. luminol, chemiluminescent substrate | Solanum lycopersicum | ? | - |
? |  |
| additional information | substrate specificity, overview, the enzyme shows broad substrate specificity and exhibits also acid phosphatase, EC 3.1.3.2 | Solanum lycopersicum | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 1 * 63000, alpha-subunit, + 1 * 57000, beta-subunit, SDS-PAGE | Solanum lycopersicum |
| More | peptide mapping | Solanum lycopersicum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Solanum lycopersicum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
- |
Solanum lycopersicum |
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Release 2023.1 (January 2023)
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