Activating Compound | Comment | Organism | Structure |
---|---|---|---|
syringaldehyde | the decolorization activity of AnaPX toward azo dyes is significantly enhanced 2-50fold in the presence of syringaldehyde | Anabaena sp. |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Anabaena sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
diethyl dicarbonate | less than 50% inhibition at 50 mM | Anabaena sp. | |
Fe2+ | 98.8% inhibition at 5 mM | Anabaena sp. | |
KCN | very low sensitivity, causes only 9% and 14% inhibition of the activity at 1 and 10 mM, respectively | Anabaena sp. | |
Mn2+ | 57.1% inhibition at 5 mM | Anabaena sp. | |
additional information | the enzyme is not sensitive to the prototypical catalase inhibitor 3-amino-1, 2, 4,-triazole in presence of ascorbic acid (1.0 mM) | Anabaena sp. | |
NaN3 | 82% inhibition 10 mM | Anabaena sp. | |
phenylhydrazine | complete inhibition at 1 mM | Anabaena sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0036 | - |
Reactive Blue 5 | at pH 4.8 | Anabaena sp. | |
0.0058 | - |
H2O2 | at pH 4.8 | Anabaena sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
53280 | - |
4 * 53280, MALDI-TOF mass spectrometry | Anabaena sp. |
53368 | - |
4 * 53368, calculated from amino acid sequence | Anabaena sp. |
54000 | - |
4 * 54000, SDS-PAGE | Anabaena sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Anabaena sp. | - |
strain PCC 7120 | - |
Oxidation Stability | Organism |
---|---|
retains more than 50% of its activity at 2.5 mM H2O2 | Anabaena sp. |
Purification (Comment) | Organism |
---|---|
DEAE-Toyopearl column chromatography, ammonium sulfate precipitation, butyl-Toyopearl column chromatography, and hydroxylapatite column chromatography | Anabaena sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
5.6 | - |
recombinant enzyme from crude extract | Anabaena sp. |
283 | - |
recombinant enzyme after 50.5fold purification | Anabaena sp. |
Storage Stability | Organism |
---|---|
4°C, in 50 mM potassium phosphate (pH 7.0), 40 days, less than 10% loss of activity | Anabaena sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,6-dimethoxyphenol + H2O2 | - |
Anabaena sp. | ? | - |
? | |
4-aminoantipyrine + H2O2 | 643% activity compared to guaiacol | Anabaena sp. | ? | - |
? | |
ABTS + H2O2 | 89% activity compared to guaiacol | Anabaena sp. | ? | - |
? | |
Acid Blue 45 + H2O2 | - |
Anabaena sp. | ? | - |
? | |
D-iso-ascorbate + H2O2 | 134% activity compared to guaiacol | Anabaena sp. | ? | - |
? | |
guaiacol + H2O2 | 100% activity | Anabaena sp. | ? | - |
? | |
L-ascorbate + H2O2 | 126% activity compared to guaiacol | Anabaena sp. | ? | - |
? | |
additional information | no activity with Mn2+ and veratryl alcohol as electron donors | Anabaena sp. | ? | - |
? | |
NADH + H2O2 | 163% activity compared to guaiacol | Anabaena sp. | ? | - |
? | |
NADPH + H2O2 | 134% activity compared to guaiacol | Anabaena sp. | ? | - |
? | |
pyrogallol + H2O2 | 93% activity compared to guaiacol | Anabaena sp. | purpurogallin + H2O | - |
? | |
Reactive Blue 114 + H2O2 | - |
Anabaena sp. | ? | - |
? | |
Reactive Blue 119 + H2O2 | - |
Anabaena sp. | ? | - |
? | |
Reactive Blue 4 + H2O2 | - |
Anabaena sp. | ? | - |
? | |
Reactive Blue 5 + H2O2 | 123% activity compared to guaiacol, the apparent optimum concentration of H2O2 required for the decolorization of Reactive Blue 5 by AnaPX is 0.4 mM | Anabaena sp. | ? | - |
? | |
syringaldehyde + H2O2 | 23% activity compared to guaiacol | Anabaena sp. | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 54000, SDS-PAGE | Anabaena sp. |
homotetramer | 4 * 53280, MALDI-TOF mass spectrometry | Anabaena sp. |
homotetramer | 4 * 53368, calculated from amino acid sequence | Anabaena sp. |
Synonyms | Comment | Organism |
---|---|---|
AnaPX | - |
Anabaena sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
- |
Anabaena sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 60 | the enzyme is considerably more stable at 30°C and 40°C where it retains more than 90% of its activity, the enzyme loses more than 90% of its activity after incubation at 50°C and 60°C for 3 h | Anabaena sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4 | 5 | - |
Anabaena sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3.5 | 9.5 | when maintained at 40°C for 20 min, the enzyme is stable at pH values between 3.5 and 9.5 | Anabaena sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | iron protoporphyrin-containing heme peroxidase | Anabaena sp. |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Anabaena sp. | isoelectric focusing | - |
3.7 |