Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | about 8fold increase in kcat value in presence of Ca2+ | Sorghum bicolor |
Crystallization (Comment) | Organism |
---|---|
to 1.27 A resolution. Metal binding sites are observed in the structure on the distal (assigned as a Na+ ion) and proximal (assigned as a Ca2+) sides of the heme | Sorghum bicolor |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.6 | - |
H2O2 | pH 5.5, 25°C | Sorghum bicolor | |
1.6 | - |
H2O2 | presence of Ca2+, pH 5.5, 25°C | Sorghum bicolor |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | enzyme contains a high-spin heme. The FeIII/FeII reduction potential is -266 mV vs normal hydrogen electrode. The ferric enzyme binds cyanide or azide to give a low-spin species | Sorghum bicolor |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sorghum bicolor | - |
- |
- |
Sorghum bicolor SK 5912 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Sorghum bicolor |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 guaiacol + H2O2 | 27% of the activity with 2,4-dichlorophenol | Sorghum bicolor | 3,3'-dimethoxy-4,4'-biphenylquinone + H2O | - |
? | |
2 guaiacol + H2O2 | 27% of the activity with 2,4-dichlorophenol | Sorghum bicolor SK 5912 | 3,3'-dimethoxy-4,4'-biphenylquinone + H2O | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
9.9 | - |
H2O2 | pH 5.5, 25°C | Sorghum bicolor | |
87 | - |
H2O2 | presence of Ca2+, pH 5.5, 25°C | Sorghum bicolor |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | enzyme contains a high-spin heme. The FeIII/FeII reduction potential is -266 mV vs normal hydrogen electrode. The gamma-heme edge shows a substrate binding site | Sorghum bicolor |