Literature summary for 1.11.1.9 extracted from

Dimastrogiovanni, D.; Anselmi, M.; Miele, A.E.; Boumis, G.; Petersson, L.; Angelucci, F.; Nola, A.D.; Brunori, M.; Bellelli, A.
Combining crystallography and molecular dynamics: The case of Schistosoma mansoni phospholipid glutathione peroxidase (2009), Proteins, 78, 259-270.

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Schistosoma mansoni

Crystallization (Commentary)

Crystallization (Comment)	Organism
sitting drop vapor diffusion method, using 0.2 M LiSO4,0.2 M sodium acetate, 24% (w/v) PEG 8000, pH 4.5 for mutant enzyme U43C and 0.2 M NaH2PO4, 0.1 M MES, 32% (w/v) PEG-MME 5000, pH 6.0 for mutant enzyme U43S	Schistosoma mansoni

Protein Variants

Protein Variants	Comment	Organism
U43C	inactive	Schistosoma mansoni
U43S	inactive	Schistosoma mansoni

Metals/Ions

Metals/Ions	Comment	Organism	Structure
selenium	Gpx is a selenium-containing enzyme	Schistosoma mansoni

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
19400	-	1 * 19400, estimated from SDS-PAGE	Schistosoma mansoni

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
H2O2 + GSH	Schistosoma mansoni	-	H2O + GSSG	-	?
tert-butyl hydroperoxide + 2 GSH	Schistosoma mansoni	-	tert-butyl alcohol + GSSG + H2O	-	?

Organism

Organism	UniProt	Comment	Textmining
Schistosoma mansoni	Q00277	-	-

Purification (Commentary)

Purification (Comment)	Organism
glutathione-Sepharose column chromatography and HiTrap SP FF column chromatography	Schistosoma mansoni

Source Tissue

Source Tissue	Comment	Organism	Textmining
integument	-	Schistosoma mansoni	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
H2O2 + GSH	-	Schistosoma mansoni	H2O + GSSG	-	?
tert-butyl hydroperoxide + 2 GSH	-	Schistosoma mansoni	tert-butyl alcohol + GSSG + H2O	-	?

Subunits

Subunits	Comment	Organism
monomer	1 * 19400, estimated from SDS-PAGE	Schistosoma mansoni

Synonyms

Synonyms	Comment	Organism
GPx4	-	Schistosoma mansoni
phospholipid glutathione peroxidase	-	Schistosoma mansoni
phospholipid hydroperoxide Gpx	-	Schistosoma mansoni

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Release 2023.1 (January 2023)