Cloned (Comment) | Organism |
---|---|
recombinant CPO and mutant enzymes S402A and F397H are overexpressed as apoenzyme in Saccharomyces cerevisiae | Curvularia inaequalis |
Protein Variants | Comment | Organism |
---|---|---|
F397H | 12% of the activity of the wild-type enzyme (chlorination of monochlorodimedone), mutant enzyme shows enhancement of bromination activity under certain conditions, inactivation of the mutant enzyme by halide especially at low pH is observed during turnover | Curvularia inaequalis |
S402A | 1.8% of the activity of wild-type enzyme (chlorination of monochlorodimedone), mutation decreases activity, mutant enzyme still catalyzes efficiently oxidation of both Cl- and Br- | Curvularia inaequalis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Br- | inhibition of mutant enzyme S402A between pH 3.5 and 4.5: concentrations greater than 0.2 mM inhibit, between pH 5.0 and 5.5: concentrations above 0.5 mM inhibit, between pH 6.0 and 6.3: 0.5 mM does not inhibit the mutant enzyme | Curvularia inaequalis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Km-value for Cl- is pH-dependent whereas the Km-value for Br- is hardly pH-dependent | Curvularia inaequalis | |
1.15 | - |
Cl- | pH 5.0, recombinant VCPO | Curvularia inaequalis | |
1.3 | - |
H2O2 | pH 6.3, mutant enzyme S402A | Curvularia inaequalis | |
2.6 | - |
H2O2 | pH 6.3, recombinant VCPO | Curvularia inaequalis | |
10.2 | - |
Cl- | pH 6.3, recombinant VCPO | Curvularia inaequalis | |
15 | - |
Br- | pH 5.0, recombinant VCPO | Curvularia inaequalis | |
15.3 | - |
Br- | pH 5.0, mutant enzyme S402A | Curvularia inaequalis | |
15.5 | - |
Cl- | pH 5.0, mutant enzyme S402A | Curvularia inaequalis | |
16.4 | - |
Cl- | pH 5.0, mutant enzyme F397H | Curvularia inaequalis | |
19.9 | - |
Br- | pH 6.3, mutant enzyme S402A | Curvularia inaequalis | |
25.8 | - |
Br- | pH 6.3, recombinant VCPO | Curvularia inaequalis | |
49.1 | - |
H2O2 | pH 5.0, recombinant VCPO | Curvularia inaequalis | |
66.7 | - |
Br- | pH 5.0, mutant enzyme F397H | Curvularia inaequalis | |
81.2 | - |
H2O2 | pH 5.0, mutant enzyme S402A | Curvularia inaequalis | |
174 | - |
Cl- | pH 6.3, mutant enzyme S402A | Curvularia inaequalis | |
177 | - |
Br- | pH pH 6.3, mutant enzyme F397H | Curvularia inaequalis | |
474 | - |
Cl- | pH pH 6.3, mutant enzyme F397H | Curvularia inaequalis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Curvularia inaequalis | P49053 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant CPO and mutant enzymes S402A and F397H are overexpressed as apoenzyme in Saccharomyces cerevisiae | Curvularia inaequalis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
monochlorodimedone + Br- + H2O2 | - |
Curvularia inaequalis | monobromo-monochlorodimedone + H2O | - |
? | |
monochlorodimedone + Cl- + H2O2 | - |
Curvularia inaequalis | dichlorodimedone + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
vanadium chloroperoxidase | - |
Curvularia inaequalis |
vCPO | - |
Curvularia inaequalis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.13 | - |
Cl- | pH 5.0, mutant enzyme S402A | Curvularia inaequalis | |
1.42 | - |
Cl- | pH 6.3, mutant enzyme S402A | Curvularia inaequalis | |
5.7 | - |
Cl- | pH pH 6.3, mutant enzyme F397H | Curvularia inaequalis | |
6.5 | - |
Cl- | pH 5.0, mutant enzyme F397H | Curvularia inaequalis | |
7.3 | - |
Cl- | pH 6.3, recombinant VCPO | Curvularia inaequalis | |
8.1 | - |
Br- | pH 6.3, mutant enzyme S402A | Curvularia inaequalis | |
15.2 | - |
H2O2 | pH 6.3, recombinant VCPO | Curvularia inaequalis | |
21.3 | - |
Br- | pH 6.3, recombinant VCPO | Curvularia inaequalis | |
23 | - |
Cl- | pH 5.0, recombinant VCPO | Curvularia inaequalis | |
33.5 | - |
Br- | pH pH 6.3, mutant enzyme F397H | Curvularia inaequalis | |
63.8 | - |
H2O2 | pH 5.0, mutant enzyme S402A | Curvularia inaequalis | |
81.7 | - |
Br- | pH 5.0, mutant enzyme S402A | Curvularia inaequalis | |
84.4 | - |
Br- | pH 5.0, mutant enzyme F397H | Curvularia inaequalis | |
183 | - |
H2O2 | pH 5.0, recombinant VCPO | Curvularia inaequalis | |
200 | - |
Br- | pH 5.0, recombinant VCPO | Curvularia inaequalis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
recombinant CVPO and mutant enzyme S402A | Curvularia inaequalis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3.5 | 5.5 | pH 3.5: about 70% of maximal activity, pH 5.5: about 45% of maximal activity | Curvularia inaequalis |