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Literature summary for 1.11.1.B2 extracted from

  • Tanaka, N.; Hasan, Z.; Wever, R.
    Kinetic characterization of active site mutants Ser402Ala and Phe397His of vanadium chloroperoxidase from the fungus Curvularia inaequalis (2003), Inorg. Chim. Acta, 356, 288-296.
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
recombinant CPO and mutant enzymes S402A and F397H are overexpressed as apoenzyme in Saccharomyces cerevisiae Curvularia inaequalis

Protein Variants

Protein Variants Comment Organism
F397H 12% of the activity of the wild-type enzyme (chlorination of monochlorodimedone), mutant enzyme shows enhancement of bromination activity under certain conditions, inactivation of the mutant enzyme by halide especially at low pH is observed during turnover Curvularia inaequalis
S402A 1.8% of the activity of wild-type enzyme (chlorination of monochlorodimedone), mutation decreases activity, mutant enzyme still catalyzes efficiently oxidation of both Cl- and Br- Curvularia inaequalis

Inhibitors

Inhibitors Comment Organism Structure
Br- inhibition of mutant enzyme S402A between pH 3.5 and 4.5: concentrations greater than 0.2 mM inhibit, between pH 5.0 and 5.5: concentrations above 0.5 mM inhibit, between pH 6.0 and 6.3: 0.5 mM does not inhibit the mutant enzyme Curvularia inaequalis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information Km-value for Cl- is pH-dependent whereas the Km-value for Br- is hardly pH-dependent Curvularia inaequalis
1.15
-
Cl- pH 5.0, recombinant VCPO Curvularia inaequalis
1.3
-
H2O2 pH 6.3, mutant enzyme S402A Curvularia inaequalis
2.6
-
H2O2 pH 6.3, recombinant VCPO Curvularia inaequalis
10.2
-
Cl- pH 6.3, recombinant VCPO Curvularia inaequalis
15
-
Br- pH 5.0, recombinant VCPO Curvularia inaequalis
15.3
-
Br- pH 5.0, mutant enzyme S402A Curvularia inaequalis
15.5
-
Cl- pH 5.0, mutant enzyme S402A Curvularia inaequalis
16.4
-
Cl- pH 5.0, mutant enzyme F397H Curvularia inaequalis
19.9
-
Br- pH 6.3, mutant enzyme S402A Curvularia inaequalis
25.8
-
Br- pH 6.3, recombinant VCPO Curvularia inaequalis
49.1
-
H2O2 pH 5.0, recombinant VCPO Curvularia inaequalis
66.7
-
Br- pH 5.0, mutant enzyme F397H Curvularia inaequalis
81.2
-
H2O2 pH 5.0, mutant enzyme S402A Curvularia inaequalis
174
-
Cl- pH 6.3, mutant enzyme S402A Curvularia inaequalis
177
-
Br- pH pH 6.3, mutant enzyme F397H Curvularia inaequalis
474
-
Cl- pH pH 6.3, mutant enzyme F397H Curvularia inaequalis

Organism

Organism UniProt Comment Textmining
Curvularia inaequalis P49053
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant CPO and mutant enzymes S402A and F397H are overexpressed as apoenzyme in Saccharomyces cerevisiae Curvularia inaequalis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
monochlorodimedone + Br- + H2O2
-
Curvularia inaequalis monobromo-monochlorodimedone + H2O
-
?
monochlorodimedone + Cl- + H2O2
-
Curvularia inaequalis dichlorodimedone + H2O
-
?

Synonyms

Synonyms Comment Organism
vanadium chloroperoxidase
-
Curvularia inaequalis
vCPO
-
Curvularia inaequalis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.13
-
Cl- pH 5.0, mutant enzyme S402A Curvularia inaequalis
1.42
-
Cl- pH 6.3, mutant enzyme S402A Curvularia inaequalis
5.7
-
Cl- pH pH 6.3, mutant enzyme F397H Curvularia inaequalis
6.5
-
Cl- pH 5.0, mutant enzyme F397H Curvularia inaequalis
7.3
-
Cl- pH 6.3, recombinant VCPO Curvularia inaequalis
8.1
-
Br- pH 6.3, mutant enzyme S402A Curvularia inaequalis
15.2
-
H2O2 pH 6.3, recombinant VCPO Curvularia inaequalis
21.3
-
Br- pH 6.3, recombinant VCPO Curvularia inaequalis
23
-
Cl- pH 5.0, recombinant VCPO Curvularia inaequalis
33.5
-
Br- pH pH 6.3, mutant enzyme F397H Curvularia inaequalis
63.8
-
H2O2 pH 5.0, mutant enzyme S402A Curvularia inaequalis
81.7
-
Br- pH 5.0, mutant enzyme S402A Curvularia inaequalis
84.4
-
Br- pH 5.0, mutant enzyme F397H Curvularia inaequalis
183
-
H2O2 pH 5.0, recombinant VCPO Curvularia inaequalis
200
-
Br- pH 5.0, recombinant VCPO Curvularia inaequalis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
recombinant CVPO and mutant enzyme S402A Curvularia inaequalis

pH Range

pH Minimum pH Maximum Comment Organism
3.5 5.5 pH 3.5: about 70% of maximal activity, pH 5.5: about 45% of maximal activity Curvularia inaequalis