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Literature summary for 1.11.1.B6 extracted from

  • Almeida, M.G.; Humanes, M.; Melo, R.; Silva, J.A.; da Silva,J.J.; Wever, R.
    Purification and characterisation of vanadium haloperoxidases from the brown alga Pelvetia canaliculata (2005), Phytochemistry, 54, 5-11.
    View publication on PubMed

General Stability

General Stability Organism
iodoperoxidase PcI is inactivated by extensive diafiltration using a Centricon-30 (Amicon) device against 100 mM citrate/phosphate pH 3.8 buffer in the presence of 1 mM EDTA, followed by a second diafiltration with 50 mM Tris-HCl (pH 9.0), reactivation by vanadium, iodoperoxidase PcI Pelvetia canaliculata
iodoperoxidase PcII is inactivated by extensive diafiltration using a Centricon-30 (Amicon) device against 100 mM citrate/phosphate pH 3.8 buffer in the presence of 1 mM EDTA, followed by a second diafiltration with 50 mM Tris-HCl (pH 9.0), reactivation by vanadium, iodoperoxidase PcI Pelvetia canaliculata

Inhibitors

Inhibitors Comment Organism Structure
I- iodoperoxidases PcII Pelvetia canaliculata

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.11
-
H2O2 iodoperoxidases PcI Pelvetia canaliculata
0.2
-
H2O2 iodoperoxidases PcII Pelvetia canaliculata
2.1
-
I- iodoperoxidases PcI Pelvetia canaliculata
2.4
-
I- iodoperoxidases PcII Pelvetia canaliculata

Metals/Ions

Metals/Ions Comment Organism Structure
vanadate essential for catalytic activity, iodoperoxidases PcI Pelvetia canaliculata
vanadate essential for catalytic activity, iodoperoxidases PcII Pelvetia canaliculata

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
-
Pelvetia canaliculata
66000
-
x * 66000, iodoperoxidases PcI, SDS-PAGE Pelvetia canaliculata
166000
-
iodoperoxidases PcI, gel filtration Pelvetia canaliculata

Organism

Organism UniProt Comment Textmining
Pelvetia canaliculata
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein iodoperoxidases PcI and iodoperoxidase PcII differ in their binding to ConA-Sepharose, which implies a different glycosylation pattern Pelvetia canaliculata

Purification (Commentary)

Purification (Comment) Organism
iodoperoxidases PcI Pelvetia canaliculata
iodoperoxidases PcII Pelvetia canaliculata

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Br- + H2O2 + 1,1-dimethyl-4-chloro-3,5-cyclohexanedione i.e. monochlorodimedone, about 5% of the iodoperoxidase activity, iodoperoxidase PcI Pelvetia canaliculata ?
-
?
Br- + H2O2 + 1,1-dimethyl-4-chloro-3,5-cyclohexanedione i.e. monochlorodimedone, about 5% of the iodoperoxidase activity, iodoperoxidase PcII Pelvetia canaliculata ?
-
?
I- + H2O2 iodoperoxidase PcI show mainly iodoperoxidase activity, the specific activity of iodoperoxidase I is half of that of iodoperoxidase II Pelvetia canaliculata triiodide
-
?
I- + H2O2 iodoperoxidase PcII shows mainly iodoperoxidase activity, the specific activity of iodoperoxidase I is half of that of iodoperoxidase II Pelvetia canaliculata triiodide
-
?

Subunits

Subunits Comment Organism
? x * 66000, iodoperoxidases PcI, SDS-PAGE Pelvetia canaliculata
More
-
Pelvetia canaliculata

Synonyms

Synonyms Comment Organism
iodoperoxidases PcI
-
Pelvetia canaliculata
iodoperoxidases PcII
-
Pelvetia canaliculata

Temperature Stability [┬░C]

Temperature Stability Minimum [┬░C] Temperature Stability Maximum [┬░C] Comment Organism
40
-
1 h, stable, iodoperoxidases PcI Pelvetia canaliculata
40
-
1 h, stable, iodoperoxidases PcII Pelvetia canaliculata
50
-
1 h, about 25% loss of activity, iodoperoxidases PcII Pelvetia canaliculata
60
-
1 h, about 50% loss of activity, iodoperoxidases PcII Pelvetia canaliculata
60
-
1 h, more than 90% loss of activity, iodoperoxidases PcI Pelvetia canaliculata
70
-
1 h, about 60% loss of activity, iodoperoxidase PcII Pelvetia canaliculata
70
-
1 h, complete inactivation, iodoperoxidases PcI Pelvetia canaliculata

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
iodoperoxidases PcI Pelvetia canaliculata
6.5
-
iodoperoxidases PcII Pelvetia canaliculata

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.06
-
I- iodoperoxidases PcII Pelvetia canaliculata
127
-
I-
-
Pelvetia canaliculata