Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.11.1.B6 extracted from

  • Almeida, M.; Filipe, S.; Humanes, M.; Maia, M.F.; Melo, R.; Severino, N.; da Silva, J.A.; Frausto da Silva, J.J.; Wever, R.
    Vanadium haloperoxidases from brown algae of the Laminariaceae family (2001), Phytochemistry, 57, 633-642.
    View publication on PubMed

General Stability

General Stability Organism
by extensive dialysis with citrate-phosphate buffer in the presence of EDTA the enzyme is inactivated due to removal of the prosthetic group Laminaria hyperborea
by extensive dialysis with citrate┬ľphosphate buffer in the presence of EDTA the enzyme is inactivated due to removal of the prosthetic group Laminaria ochroleuca

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.12
-
H2O2 pH 6.7, isoenzyme Ls1 Laminaria ochroleuca
0.137
-
H2O2 pH 6.7, isoenzyme Ls2 Laminaria ochroleuca
0.166
-
H2O2 pH 6.7, isoenzyme Lh1 Laminaria hyperborea
0.173
-
H2O2 pH 6.7, isoenzyme Lh2 Laminaria hyperborea
0.217
-
H2O2 pH 6.1, isoenzyme Lh2 Laminaria hyperborea
0.243
-
H2O2 pH 6.1, isoenzyme Ls1 Laminaria ochroleuca
0.273
-
H2O2 pH 6.1, isoenzyme Ls2 Laminaria ochroleuca
0.275
-
H2O2 pH 6.1, isoenzyme Lh1 Laminaria hyperborea
0.285
-
H2O2 pH 5.5, isoenzyme Lh2 Laminaria hyperborea
0.333
-
H2O2 pH 5.5, isoenzyme Ls2 Laminaria ochroleuca
0.334
-
H2O2 pH 5.5, isoenzyme Lh1 Laminaria hyperborea
0.376
-
H2O2 pH 5.5, isoenzyme Ls1 Laminaria ochroleuca
1.3
-
I- pH 5.5, isoenzyme Ls1 Laminaria ochroleuca
1.9
-
I- pH 5.5, isoenzyme Lh1 Laminaria hyperborea
1.9
-
I- pH 5.5, isoenzyme Ls2 Laminaria ochroleuca
2.3
-
I- pH 5.5, isoenzyme Lh2 Laminaria hyperborea
2.7
-
I- pH 6.1, isoenzyme Ls2 Laminaria ochroleuca
3.4
-
I- pH 6.1, isoenzyme Lh1 Laminaria hyperborea
3.4
-
I- pH 6.1, isoenzyme Ls1 Laminaria ochroleuca
3.5
-
I- pH 6.7, isoenzyme Lh1 Laminaria hyperborea
3.7
-
I- pH 6.7, isoenzyme Ls1 Laminaria ochroleuca
3.8
-
I- pH 6.1, isoenzyme Lh2 Laminaria hyperborea
3.8
-
I- pH 6.7, isoenzyme Lh2 Laminaria hyperborea
4.3
-
I- pH 6.7, isoenzyme Ls2 Laminaria ochroleuca

Metals/Ions

Metals/Ions Comment Organism Structure
Vanadium enzyme contains vanadium Laminaria ochroleuca
Vanadium the enzyme contains vanadium Laminaria hyperborea

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
65000
-
x * 65000, SDS-PAGE Laminaria ochroleuca
67000
-
x * 67000, SDS-PAGE Laminaria hyperborea
67000
-
x * 67000, SDS-PAGE Laminaria ochroleuca

Organic Solvent Stability

Organic Solvent Comment Organism
1-propanol 50% v/v, 30 day incubation, about 70% loss of activity of isoenzyme Lh2 Laminaria hyperborea
1-propanol 50% v/v, 30 day incubation, more than 95% loss of activity of isoenzyme Ls1, about 75% loss of activity of isoenzyme Ls2 Laminaria ochroleuca
Acetone 50% v/v, 30 day incubation, about 80% loss of activity of isoenzyme Lh2 Laminaria hyperborea
Acetone 50% v/v, 30 day incubation, more than 90% loss of activity of isoenzyme Ls1 and Ls2 Laminaria ochroleuca
Ethanol 50% v/v, 30 day incubation, about 90% loss of activity of isoenzyme Lh2 Laminaria hyperborea
Ethanol 50% v/v, 30 day incubation, more than 90% loss of activity of isoenzyme Ls1 and Ls2 Laminaria ochroleuca
Methanol 50% v/v, 30 day incubation, about 70% loss of activity of isoenzyme Lh2 Laminaria hyperborea
Methanol 50% v/v, 30 day incubation, more than 90% loss of activity of isoenzyme Ls1 and Ls2 Laminaria ochroleuca

Organism

Organism UniProt Comment Textmining
Laminaria hyperborea
-
-
-
Laminaria ochroleuca
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Br- + H2O2 + 1,1-dimethyl-4-chloro-3,5-cyclohexanedione i.e. monochlorodimedone. Isoenzyme Lh2 shows 5% of the activity with iodide + H2O2, isoenzyme Lh1 is inactive Laminaria hyperborea ?
-
?
I- + H2O2
-
Laminaria hyperborea triiodide + ?
-
?
I- + H2O2
-
Laminaria ochroleuca triiodide + ?
-
?
additional information no monochlorodimedonebromination activity Laminaria ochroleuca ?
-
?

Subunits

Subunits Comment Organism
? x * 67000, SDS-PAGE Laminaria hyperborea
? x * 67000, SDS-PAGE Laminaria ochroleuca
? x * 65000, SDS-PAGE Laminaria ochroleuca

Temperature Optimum [┬░C]

Temperature Optimum [┬░C] Temperature Optimum Maximum [┬░C] Comment Organism
40
-
isoenzyme Ls1 Laminaria ochroleuca

Temperature Stability [┬░C]

Temperature Stability Minimum [┬░C] Temperature Stability Maximum [┬░C] Comment Organism
30 50 stable Laminaria hyperborea
30 50 stable Laminaria ochroleuca