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Literature summary for 1.11.1.B6 extracted from

  • Almeida, M.; Filipe, S.; Humanes, M.; Maia, M.F.; Melo, R.; Severino, N.; da Silva, J.A.; Frausto da Silva, J.J.; Wever, R.
    Vanadium haloperoxidases from brown algae of the Laminariaceae family (2001), Phytochemistry, 57, 633-642.
    View publication on PubMed
Search for more literature for 1.11.1.B6

General Stability

General Stability Organism
by extensive dialysis with citrate-phosphate buffer in the presence of EDTA the enzyme is inactivated due to removal of the prosthetic group Laminaria hyperborea
by extensive dialysis with citrate–phosphate buffer in the presence of EDTA the enzyme is inactivated due to removal of the prosthetic group Laminaria ochroleuca

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.12
-
 H2O2 pH 6.7, isoenzyme Ls1 Laminaria ochroleuca
0.137
-
 H2O2 pH 6.7, isoenzyme Ls2 Laminaria ochroleuca
0.166
-
 H2O2 pH 6.7, isoenzyme Lh1 Laminaria hyperborea
0.173
-
 H2O2 pH 6.7, isoenzyme Lh2 Laminaria hyperborea
0.217
-
 H2O2 pH 6.1, isoenzyme Lh2 Laminaria hyperborea
0.243
-
 H2O2 pH 6.1, isoenzyme Ls1 Laminaria ochroleuca
0.273
-
 H2O2 pH 6.1, isoenzyme Ls2 Laminaria ochroleuca
0.275
-
 H2O2 pH 6.1, isoenzyme Lh1 Laminaria hyperborea
0.285
-
 H2O2 pH 5.5, isoenzyme Lh2 Laminaria hyperborea
0.333
-
 H2O2 pH 5.5, isoenzyme Ls2 Laminaria ochroleuca
0.334
-
 H2O2 pH 5.5, isoenzyme Lh1 Laminaria hyperborea
0.376
-
 H2O2 pH 5.5, isoenzyme Ls1 Laminaria ochroleuca
1.3
-
 I- pH 5.5, isoenzyme Ls1 Laminaria ochroleuca
1.9
-
 I- pH 5.5, isoenzyme Lh1 Laminaria hyperborea
1.9
-
 I- pH 5.5, isoenzyme Ls2 Laminaria ochroleuca
2.3
-
 I- pH 5.5, isoenzyme Lh2 Laminaria hyperborea
2.7
-
 I- pH 6.1, isoenzyme Ls2 Laminaria ochroleuca
3.4
-
 I- pH 6.1, isoenzyme Lh1 Laminaria hyperborea
3.4
-
 I- pH 6.1, isoenzyme Ls1 Laminaria ochroleuca
3.5
-
 I- pH 6.7, isoenzyme Lh1 Laminaria hyperborea
3.7
-
 I- pH 6.7, isoenzyme Ls1 Laminaria ochroleuca
3.8
-
 I- pH 6.1, isoenzyme Lh2 Laminaria hyperborea
3.8
-
 I- pH 6.7, isoenzyme Lh2 Laminaria hyperborea
4.3
-
 I- pH 6.7, isoenzyme Ls2 Laminaria ochroleuca

Metals/Ions

Metals/Ions Comment Organism Structure
Vanadium enzyme contains vanadium Laminaria ochroleuca
Vanadium the enzyme contains vanadium Laminaria hyperborea

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
65000
-
 x * 65000, SDS-PAGE Laminaria ochroleuca
67000
-
 x * 67000, SDS-PAGE Laminaria hyperborea
67000
-
 x * 67000, SDS-PAGE Laminaria ochroleuca

Organic Solvent Stability

Organic Solvent Comment Organism
1-propanol 50% v/v, 30 day incubation, about 70% loss of activity of isoenzyme Lh2 Laminaria hyperborea
1-propanol 50% v/v, 30 day incubation, more than 95% loss of activity of isoenzyme Ls1, about 75% loss of activity of isoenzyme Ls2 Laminaria ochroleuca
Acetone 50% v/v, 30 day incubation, about 80% loss of activity of isoenzyme Lh2 Laminaria hyperborea
Acetone 50% v/v, 30 day incubation, more than 90% loss of activity of isoenzyme Ls1 and Ls2 Laminaria ochroleuca
Ethanol 50% v/v, 30 day incubation, about 90% loss of activity of isoenzyme Lh2 Laminaria hyperborea
Ethanol 50% v/v, 30 day incubation, more than 90% loss of activity of isoenzyme Ls1 and Ls2 Laminaria ochroleuca
Methanol 50% v/v, 30 day incubation, about 70% loss of activity of isoenzyme Lh2 Laminaria hyperborea
Methanol 50% v/v, 30 day incubation, more than 90% loss of activity of isoenzyme Ls1 and Ls2 Laminaria ochroleuca

Organism

Organism UniProt Comment Textmining
Laminaria hyperborea
-
-
-
Laminaria ochroleuca
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Br- + H2O2 + 1,1-dimethyl-4-chloro-3,5-cyclohexanedione i.e. monochlorodimedone. Isoenzyme Lh2 shows 5% of the activity with iodide + H2O2, isoenzyme Lh1 is inactive Laminaria hyperborea ?
-
 ?
I- + H2O2
-
 Laminaria hyperborea triiodide + ?
-
 ?
I- + H2O2
-
 Laminaria ochroleuca triiodide + ?
-
 ?
additional information no monochlorodimedonebromination activity Laminaria ochroleuca ?
-
 ?

Subunits

Subunits Comment Organism
? x * 67000, SDS-PAGE Laminaria hyperborea
? x * 67000, SDS-PAGE Laminaria ochroleuca
? x * 65000, SDS-PAGE Laminaria ochroleuca

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
 isoenzyme Ls1 Laminaria ochroleuca

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30 50 stable Laminaria hyperborea
30 50 stable Laminaria ochroleuca