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Literature summary for 1.11.1.B6 extracted from

  • Fournier, J.B.; Rebuffet, E.; Delage, L.; Grijol, R.; Meslet-Cladiere, L.; Rzonca, J.; Potin, P.; Michel, G.; Czjzek, M.; Leblanc, C.
    The vanadium iodoperoxidase from the marine flavobacteriaceae species Zobellia galactanivorans reveals novel molecular and evolutionary features of halide specificity in the vanadium haloperoxidase enzyme family (2014), Appl. Environ. Microbiol., 80, 7561-7573 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Zobellia galactanivorans

Crystallization (Commentary)

Crystallization (Comment) Organism
to 1.8 A resolution, revealing a monomeric structure mainly folded into alpha-helices. The vanadate binding site is strictly conserved with the fungal VCPO active site. Specific amino acids and the associated hydrogen bonding network around the vanadate center are essential for the catalytic properties and also the iodide specificity Zobellia galactanivorans

Protein Variants

Protein Variants Comment Organism
C320S 34% of wild-type activity Zobellia galactanivorans
D322L 31% of wild-type activity Zobellia galactanivorans
D322Y 0.3% of wild-type activity Zobellia galactanivorans
F353H 225% of wild-type activity Zobellia galactanivorans
H360A 6% of wild-type activity Zobellia galactanivorans
H360S 0.4% of wild-type activity Zobellia galactanivorans
R410A 0.1% of wild-type activity Zobellia galactanivorans
S358A 128% of wild-type activity Zobellia galactanivorans
W321R 51% of wild-type activity Zobellia galactanivorans
Y263A 89% of wild-type activity Zobellia galactanivorans
Y263F 38% of wild-type activity Zobellia galactanivorans
Y263S 98% of wild-type activity Zobellia galactanivorans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.02
-
thymol blue mutant H360A, pH 7.2, 20┬░C Zobellia galactanivorans
0.03
-
thymol blue mutant D322Y, pH 7.2, 20┬░C Zobellia galactanivorans
0.05
-
thymol blue mutant H360S, pH 7.2, 20┬░C Zobellia galactanivorans
0.19
-
thymol blue mutant S358A, pH 7.2, 20┬░C Zobellia galactanivorans
0.22
-
thymol blue wild-type, pH 7.2, 20┬░C Zobellia galactanivorans
0.39
-
thymol blue mutant W321R, pH 7.2, 20┬░C Zobellia galactanivorans
0.44
-
thymol blue mutant Y263A, pH 7.2, 20┬░C Zobellia galactanivorans
0.79
-
thymol blue mutant Y263F, pH 7.2, 20┬░C Zobellia galactanivorans
0.91
-
thymol blue mutant C320S, pH 7.2, 20┬░C Zobellia galactanivorans
1.38
-
thymol blue mutant D322L, pH 7.2, 20┬░C Zobellia galactanivorans
1.58
-
thymol blue mutant Y263S, pH 7.2, 20┬░C Zobellia galactanivorans
2.63
-
thymol blue mutant F353H, pH 7.2, 20┬░C Zobellia galactanivorans
3.45
-
thymol blue mutant R410A, pH 7.2, 20┬░C Zobellia galactanivorans

Organism

Organism UniProt Comment Textmining
Zobellia galactanivorans G0LAH5
-
-
Zobellia galactanivorans DSM 12802 G0LAH5
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information enzyme shows strict specificity for iodide oxidation Zobellia galactanivorans ?
-
?
additional information enzyme shows strict specificity for iodide oxidation Zobellia galactanivorans DSM 12802 ?
-
?
thymol blue + 2 I- + 2 H+ + H2O2
-
Zobellia galactanivorans diiodothymol blue + 2 H2O
-
?
thymol blue + 2 I- + 2 H+ + H2O2
-
Zobellia galactanivorans DSM 12802 diiodothymol blue + 2 H2O
-
?

Synonyms

Synonyms Comment Organism
zobellia_1262
-
Zobellia galactanivorans

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0007
-
thymol blue mutant D322Y, pH 7.2, 20┬░C Zobellia galactanivorans
0.002
-
thymol blue mutant H360S, pH 7.2, 20┬░C Zobellia galactanivorans
0.01
-
thymol blue mutant H360A, pH 7.2, 20┬░C Zobellia galactanivorans
0.03
-
thymol blue mutant R410A, pH 7.2, 20┬░C Zobellia galactanivorans
1.78
-
thymol blue mutant W321R, pH 7.2, 20┬░C Zobellia galactanivorans
1.98
-
thymol blue wild-type, pH 7.2, 20┬░C Zobellia galactanivorans
2.19
-
thymol blue mutant S358A, pH 7.2, 20┬░C Zobellia galactanivorans
2.67
-
thymol blue mutant Y263F, pH 7.2, 20┬░C Zobellia galactanivorans
2.76
-
thymol blue mutant C320S, pH 7.2, 20┬░C Zobellia galactanivorans
3.53
-
thymol blue mutant Y263A, pH 7.2, 20┬░C Zobellia galactanivorans
3.86
-
thymol blue mutant D322L, pH 7.2, 20┬░C Zobellia galactanivorans
13.97
-
thymol blue mutant Y263S, pH 7.2, 20┬░C Zobellia galactanivorans
53.27
-
thymol blue mutant F353H, pH 7.2, 20┬░C Zobellia galactanivorans

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.01
-
thymol blue mutant R410A, pH 7.2, 20┬░C Zobellia galactanivorans
0.02
-
thymol blue mutant D322Y, pH 7.2, 20┬░C Zobellia galactanivorans
0.04
-
thymol blue mutant H360S, pH 7.2, 20┬░C Zobellia galactanivorans
0.5
-
thymol blue mutant H360A, pH 7.2, 20┬░C Zobellia galactanivorans
2.8
-
thymol blue mutant D322L, pH 7.2, 20┬░C Zobellia galactanivorans
3.03
-
thymol blue mutant C320S, pH 7.2, 20┬░C Zobellia galactanivorans
3.38
-
thymol blue mutant Y263F, pH 7.2, 20┬░C Zobellia galactanivorans
4.56
-
thymol blue mutant W321R, pH 7.2, 20┬░C Zobellia galactanivorans
8.02
-
thymol blue mutant Y263A, pH 7.2, 20┬░C Zobellia galactanivorans
8.84
-
thymol blue mutant Y263S, pH 7.2, 20┬░C Zobellia galactanivorans
9
-
thymol blue wild-type, pH 7.2, 20┬░C Zobellia galactanivorans
11.53
-
thymol blue mutant S358A, pH 7.2, 20┬░C Zobellia galactanivorans
20.25
-
thymol blue mutant F353H, pH 7.2, 20┬░C Zobellia galactanivorans