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Literature summary for 1.11.2.1 extracted from

  • Ullrich, R.; Dolge, C.; Kluge, M.; Hofrichter, M.
    Pyridine as novel substrate for regioselective oxygenation with aromatic peroxygenase from Agrocybe aegerita (2008), FEBS Lett., 582, 4100-4106.
    View publication on PubMed
Search for more literature for 1.11.2.1

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.069
-
 Pyridine in 10 mM phosphate buffer (pH 7.0), temperature not specified in the publication Cyclocybe aegerita

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Cyclocybe aegerita
-
-

Organism

Organism UniProt Comment Textmining
Cyclocybe aegerita
-
 synonym Agrocybe cylindrical, strain A 1
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
75
-
 isoform P II, using veratryl alcohol as substrate, pH 7.0, temperature not specified in the publication Cyclocybe aegerita

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-chloropyridine + H2O2 26.1% conversion compared to pyridine Cyclocybe aegerita 2-chloropyridine N-oxide + H2O
-
 ?
3,5-dimethylpyridine + H2O2 143.4% conversion compared to pyridine Cyclocybe aegerita 5-methyl-nicotinic alcohol + 5-methyl-nicotinic aldehyde + 3,5-dimethylpyridine N-oxide + H2O 3,5-dimethylpyridine N-oxide is less than 1% of the converted substrate ?
3-bromopyridine + H2O2 61.8% conversion compared to pyridine Cyclocybe aegerita 3-bromopyridine N-oxide + H2O
-
 ?
3-chloropyridine + H2O2 47.2% conversion compared to pyridine Cyclocybe aegerita 3-chloropyridine N-oxide + nicotinic alcohol + nicotinic aldehyde + nicotinic acid + H2O
-
 ?
3-cyanopyridine + H2O2 moderate substrate with 25.4% conversion compared to pyridine Cyclocybe aegerita 3-cyanopyridine N-oxide + H2O
-
 ?
3-fluoropyridine + H2O2 39.4% conversion compared to pyridine Cyclocybe aegerita 3-fluoropyridine N-oxide + H2O
-
 ?
3-iodopyridine + H2O2 3-iodopyridine is slightly better oxidized than unsubstituted pyridine (102.2% conversion) Cyclocybe aegerita 3-iodopyridine N-oxide + H2O
-
 ?
3-methylpyridine + H2O2 98.4% conversion compared to pyridine Cyclocybe aegerita 3-methylpyridine N-oxide + H2O
-
 ?
3-nitropyridine + H2O2 moderate substrate with 5.4% conversion compared to pyridine Cyclocybe aegerita 3-nitropyridine N-oxide + H2O
-
 ?
4-chloropyridine + H2O2 4-chloropyridine is slightly better oxidized than unsubstituted pyridine (102.9% conversion) Cyclocybe aegerita 4-chloropyridine N-oxide + H2O
-
 ?
additional information nicotinic acid, nicotine amide, 3,5-dichloropyridine and perchloropyridine are no substrates Cyclocybe aegerita ?
-
 ?
pyridine + H2O2 100% conversion Cyclocybe aegerita pyridine N-oxide + H2O
-
 ?
veratryl alcohol + H2O2 + H+
-
 Cyclocybe aegerita veratraldehyde + H2O
-
 ?

Synonyms

Synonyms Comment Organism
aromatic peroxygenase
-
 Cyclocybe aegerita
haloperoxidase
-
 Cyclocybe aegerita
haloperoxidase-peroxygenase
-
 Cyclocybe aegerita

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.21
-
 Pyridine in 10 mM phosphate buffer (pH 7.0), temperature not specified in the publication Cyclocybe aegerita

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Cyclocybe aegerita

pH Range

pH Minimum pH Maximum Comment Organism
4 11
-
 Cyclocybe aegerita

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.04
-
 Pyridine in 10 mM phosphate buffer (pH 7.0), temperature not specified in the publication Cyclocybe aegerita