Literature summary for 1.11.2.1 extracted from

Hayakawa, S.; Matsumura, H.; Nakamura, N.; Yohda, M.; Ohno, H.
Identification of the rate-limiting step of the peroxygenase reactions catalyzed by the thermophilic cytochrome P450 from Sulfolobus tokodaii strain 7 (2014), FEBS J., 281, 1409-1416.

Search for more literature for 1.11.2.1

Protein Variants

Protein Variants	Comment	Organism
F310A/A320Q	specific activity of peroxygenase is significantly increased over a broad pH-range. The pH dependence of the reaction is altered to bell shaped, with maximum activities near pH 5. At pH 5, the mutant is over 15 times more active than wild-type P450st	Sulfurisphaera tokodaii

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
7.2	-	H2O2	pH 5, 30°C, mutant enzyme F310A/F320Q	Sulfurisphaera tokodaii
9.3	-	H2O2	pH 10, 30°C, mutant enzyme F310A/F320Q	Sulfurisphaera tokodaii
23.2	-	H2O2	pH 5, 30°C, wild-type enzyme	Sulfurisphaera tokodaii
26.2	-	H2O2	pH 10, 30°C, wild-type enzyme	Sulfurisphaera tokodaii
30.6	-	H2O2	pH 7, 30°C, mutant enzyme F310A/F320Q	Sulfurisphaera tokodaii
81.4	-	H2O2	pH 7, 30°C, wild-type enzyme	Sulfurisphaera tokodaii

Organism

Organism	UniProt	Comment	Textmining
Sulfurisphaera tokodaii	-	-	-
Sulfurisphaera tokodaii 7	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ethylbenzene + H2O2	-	Sulfurisphaera tokodaii	1-phenylethanol + H2O	1-phenylethanol is the major product	?
ethylbenzene + H2O2	-	Sulfurisphaera tokodaii 7	1-phenylethanol + H2O	1-phenylethanol is the major product	?

Synonyms

Synonyms	Comment	Organism
P450st	-	Sulfurisphaera tokodaii

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.07	-	H2O2	pH 5, 30°C, mutant enzyme F310A/F320Q	Sulfurisphaera tokodaii
0.09	-	H2O2	pH 5, 30°C, wild-type enzyme	Sulfurisphaera tokodaii
0.09	-	H2O2	pH 10, 30°C, mutant enzyme F310A/F320Q	Sulfurisphaera tokodaii
0.13	-	H2O2	pH 7, 30°C, wild-type enzyme	Sulfurisphaera tokodaii
0.16	-	H2O2	pH 10, 30°C, wild-type enzyme	Sulfurisphaera tokodaii
0.34	-	H2O2	pH 7, 30°C, mutant enzyme F310A/F320Q	Sulfurisphaera tokodaii

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7	-	-	Sulfurisphaera tokodaii

pH Range

pH Minimum	pH Maximum	Comment	Organism
6	9	pH 6.0: about 60% of maximal activity, pH 9.0: about 45% of maximal activity	Sulfurisphaera tokodaii

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
0.0016	-	H2O2	pH 7, 30°C, wild-type enzyme	Sulfurisphaera tokodaii
0.004	-	H2O2	pH 5, 30°C, wild-type enzyme	Sulfurisphaera tokodaii
0.006	-	H2O2	pH 10, 30°C, wild-type enzyme	Sulfurisphaera tokodaii
0.0096	-	H2O2	pH 10, 30°C, mutant enzyme F310A/F320Q	Sulfurisphaera tokodaii
0.0097	-	H2O2	pH 5, 30°C, mutant enzyme F310A/F320Q	Sulfurisphaera tokodaii
0.011	-	H2O2	pH 7, 30°C, mutant enzyme F310A/F320Q	Sulfurisphaera tokodaii

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Release 2023.1 (January 2023)