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Literature summary for 1.11.2.1 extracted from
- Tandem-yeast expression system for engineering and producing unspecific peroxygenase (2015), Enzyme Microb. Technol., 73-74, 29-33 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Pichia pastoris and Saccharomyces cerevisiae | Cyclocybe aegerita |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.048 | - |
2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) | recombinant enzyme from Saccharomyces cerevisiae, at pH 4.0, temperature not specified in the publication | Cyclocybe aegerita |  |
| 0.05 | - |
2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) | recombinant enzyme from Pichia pastoris, at pH 4.0, temperature not specified in the publication | Cyclocybe aegerita | |
| 0.483 | - |
5-nitro-1,3-benzodioxole | recombinant enzyme from Saccharomyces cerevisiae, at pH 7.0, temperature not specified in the publication | Cyclocybe aegerita | |
| 0.49 | - |
H2O2 | recombinant enzyme from Saccharomyces cerevisiae, with benzyl alcohol as cosubstrate, at pH 7.0, temperature not specified in the publication | Cyclocybe aegerita | |
| 0.85 | - |
5-nitro-1,3-benzodioxole | recombinant enzyme from Pichia pastoris, at pH 7.0, temperature not specified in the publication | Cyclocybe aegerita | |
| 1.53 | - |
H2O2 | recombinant enzyme from Pichia pastoris, with benzyl alcohol as cosubstrate, at pH 7.0, temperature not specified in the publication | Cyclocybe aegerita | |
| 2.5 | - |
benzyl alcohol | recombinant enzyme from Saccharomyces cerevisiae, at pH 7.0 temperature not specified in the publication | Cyclocybe aegerita | |
| 2.8 | - |
benzyl alcohol | recombinant enzyme from Pichia pastoris, at pH 7.0 temperature not specified in the publication | Cyclocybe aegerita | |
| 6.2 | - |
veratryl alcohol | recombinant enzyme from Pichia pastoris, at pH 7.0, temperature not specified in the publication | Cyclocybe aegerita | |
| 7.9 | - |
veratryl alcohol | recombinant enzyme from Saccharomyces cerevisiae, at pH 7.0, temperature not specified in the publication | Cyclocybe aegerita | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cyclocybe aegerita | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | - |
Cyclocybe aegerita |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate precipitation, Phenyl Sepharose column chromatography, and Q-Source column chromatography | Cyclocybe aegerita |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O2 | - |
Cyclocybe aegerita | oxidized 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O | - |
? | |
| 5-nitro-1,3-benzodioxole + H2O2 | - |
Cyclocybe aegerita | 4-nitrocatechol + formic acid + H2O | - |
? | |
| benzyl alcohol + H2O2 | - |
Cyclocybe aegerita | benzaldehyde + H2O | - |
? | |
| veratryl alcohol + H2O2 | - |
Cyclocybe aegerita | veratraldehyde + H2O | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 52000, SDS-PAGE | Cyclocybe aegerita |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| aromatic peroxygenase | - |
Cyclocybe aegerita |
| UPO | - |
Cyclocybe aegerita |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 121 | - |
veratryl alcohol | recombinant enzyme from Saccharomyces cerevisiae, at pH 7.0, temperature not specified in the publication | Cyclocybe aegerita | |
| 203 | - |
veratryl alcohol | recombinant enzyme from Pichia pastoris, at pH 7.0, temperature not specified in the publication | Cyclocybe aegerita | |
| 238 | - |
H2O2 | recombinant enzyme from Saccharomyces cerevisiae, with benzyl alcohol as cosubstrate, at pH 7.0, temperature not specified in the publication | Cyclocybe aegerita | |
| 307 | - |
benzyl alcohol | recombinant enzyme from Saccharomyces cerevisiae, at pH 7.0 temperature not specified in the publication | Cyclocybe aegerita | |
| 338 | - |
5-nitro-1,3-benzodioxole | recombinant enzyme from Saccharomyces cerevisiae, at pH 7.0, temperature not specified in the publication | Cyclocybe aegerita | |
| 395 | - |
2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) | recombinant enzyme from Saccharomyces cerevisiae, at pH 4.0, temperature not specified in the publication | Cyclocybe aegerita | |
| 498 | - |
5-nitro-1,3-benzodioxole | recombinant enzyme from Pichia pastoris, at pH 7.0, temperature not specified in the publication | Cyclocybe aegerita | |
| 524 | - |
benzyl alcohol | recombinant enzyme from Pichia pastoris, at pH 7.0 temperature not specified in the publication | Cyclocybe aegerita | |
| 546 | - |
2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) | recombinant enzyme from Pichia pastoris, at pH 4.0, temperature not specified in the publication | Cyclocybe aegerita | |
| 676 | - |
H2O2 | recombinant enzyme from Pichia pastoris, with benzyl alcohol as cosubstrate, at pH 7.0, temperature not specified in the publication | Cyclocybe aegerita | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| heme | - |
Cyclocybe aegerita | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 15 | - |
veratryl alcohol | recombinant enzyme from Saccharomyces cerevisiae, at pH 7.0, temperature not specified in the publication | Cyclocybe aegerita | |
| 32 | - |
veratryl alcohol | recombinant enzyme from Pichia pastoris, at pH 7.0, temperature not specified in the publication | Cyclocybe aegerita | |
| 124 | - |
benzyl alcohol | recombinant enzyme from Saccharomyces cerevisiae, at pH 7.0 temperature not specified in the publication | Cyclocybe aegerita | |
| 190 | - |
benzyl alcohol | recombinant enzyme from Pichia pastoris, at pH 7.0 temperature not specified in the publication | Cyclocybe aegerita | |
| 442 | - |
H2O2 | recombinant enzyme from Pichia pastoris, with benzyl alcohol as cosubstrate, at pH 7.0, temperature not specified in the publication | Cyclocybe aegerita | |
| 500 | - |
H2O2 | recombinant enzyme from Saccharomyces cerevisiae, with benzyl alcohol as cosubstrate, at pH 7.0, temperature not specified in the publication | Cyclocybe aegerita | |
| 590 | - |
5-nitro-1,3-benzodioxole | recombinant enzyme from Pichia pastoris, at pH 7.0, temperature not specified in the publication | Cyclocybe aegerita | |
| 700 | - |
5-nitro-1,3-benzodioxole | recombinant enzyme from Saccharomyces cerevisiae, at pH 7.0, temperature not specified in the publication | Cyclocybe aegerita | |
| 8200 | - |
2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) | recombinant enzyme from Saccharomyces cerevisiae, at pH 4.0, temperature not specified in the publication | Cyclocybe aegerita | |
| 11000 | - |
2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) | recombinant enzyme from Pichia pastoris, at pH 4.0, temperature not specified in the publication | Cyclocybe aegerita | |
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Release 2023.1 (January 2023)
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