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Literature summary for 1.11.2.2 extracted from

  • van Dalen, C.J.; Whitehouse, M.W.; Winterbourn, C.C.; Kettle, A.J.
    Thiocyanate and chloride as competing substrates for myeloperoxidase (1997), Biochem. J., 327, 487-492.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.11.2.2

Inhibitors

Inhibitors Comment Organism Structure
5-thio-2-nitrobenzoic acid competitive inhibitor of myeloperoxidase Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.5
-
 thiocyanate in 100 mM phosphate buffer, pH 7.4, at 21°C Homo sapiens
2
-
 Br- in 100 mM phosphate buffer, pH 7.4, at 21°C Homo sapiens
175
-
 Cl- in 100 mM phosphate buffer, pH 7.4, at 21°C Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
15500
-
 SDS-PAGE under reducing conditions gives three bands with molecular masses of 57000, 39000 and 15000 Da, respectively Homo sapiens
39000
-
 SDS-PAGE under reducing conditions gives three bands with molecular masses of 57000, 39000 and 15000 Da, respectively Homo sapiens
57000
-
 SDS-PAGE under reducing conditions gives three bands with molecular masses of 57000, 39000 and 15000 Da, respectively Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Cl- + H2O2 + H+ Homo sapiens physiological substrate of myeloperoxidase HClO + H2O
-
 ?
thiocyanate + H2O2 + H+ Homo sapiens thiocyanate is a major physiological substrate of myeloperoxidase and most favoured substrate compared to chloride and bromide hypothiocyanite + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
leukocyte
-
 Homo sapiens
-
neutrophil
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Br- + H2O2 + H+
-
 Homo sapiens HBrO + H2O
-
 ?
Cl- + H2O2 + H+
-
 Homo sapiens HClO + H2O
-
 ?
Cl- + H2O2 + H+ physiological substrate of myeloperoxidase Homo sapiens HClO + H2O
-
 ?
I- + H2O2 + H+
-
 Homo sapiens HIO + H2O
-
 ?
thiocyanate + H2O2 + H+ thiocyanate is a major physiological substrate of myeloperoxidase and most favoured substrate compared to chloride and bromide Homo sapiens hypothiocyanite + H2O
-
 ?
thiocyanate + H2O2 + H+ thiocyanate is the most favoured substrate compared to chloride and bromide. With 0.1 mM thiocyanate about 50% of the H2O2 present is converted into hypothiocyanite, the rate of H2O2 loss catalysed by myeloperoxidase in the presence of 100 mM chloride doubles when 0.1 mM thiocyanate is added, and is maximal with 1 mM thiocyanate Homo sapiens hypothiocyanite + H2O
-
 ?

Synonyms

Synonyms Comment Organism
MPO
-
 Homo sapiens

Cofactor

Cofactor Comment Organism Structure
heme
-
 Homo sapiens