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Literature summary for 1.11.2.4 extracted from
- Functional modulation of a peroxygenase cytochrome P450: novel insight into the mechanisms of peroxygenase and peroxidase enzymes (2002), FEBS Lett., 528, 90-94.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Bacillus subtilis |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| R242K | the mutant enzyme bound with deuterated myristic acid shows greatly increased rate of 3,5,3',5'-tetramethylbenzidine oxidation | Bacillus subtilis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 3,5,3',5'-tetramethylbenzidine | 3,5,3',5'-tetramethylbenzidine as an additional substrate intercepts the hydroxylation of myristic acid | Bacillus subtilis |  |
| myristic acid | myristic acid hydroxylation is inhibited by 3,5,3',5'-tetramethylbenzidine oxidation in a 3,5,3',5'-tetramethylbenzidine concentration-dependent manner | Bacillus subtilis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.026 | - |
myristic acid | pH and temperature not specified in the publication | Bacillus subtilis | |
| 0.026 | - |
3,5,3',5'-tetramethylbenzidine | pH and temperature not specified in the publication | Bacillus subtilis | |
| 0.035 | - |
H2O2 | pH and temperature not specified in the publication | Bacillus subtilis | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3,5,3',5'-tetramethylbenzidine + H2O2 | Bacillus subtilis | a myristic acid-dependent reaction, when deuterated myristic acid is used as a substrate to decrease hydroxylation activity, the rate of 3,5,3',5'-tetramethylbenzidine oxidation increases | ? | - |
? | |
| additional information | Bacillus subtilis | a reductase and NAD(P)H are not required for activity | ? | - |
? | |
| myristic acid + H2O2 | Bacillus subtilis | - |
? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus subtilis | O31440 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bacillus subtilis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3,5,3',5'-tetramethylbenzidine + H2O2 | a myristic acid-dependent reaction, when deuterated myristic acid is used as a substrate to decrease hydroxylation activity, the rate of 3,5,3',5'-tetramethylbenzidine oxidation increases | Bacillus subtilis | ? | - |
? | |
| additional information | a reductase and NAD(P)H are not required for activity | Bacillus subtilis | ? | - |
? | |
| myristic acid + H2O2 | - |
Bacillus subtilis | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CYP152A1 | - |
Bacillus subtilis |
| cytochrome P450BSbeta | - |
Bacillus subtilis |
| peroxygenase cytochrome P450 | - |
Bacillus subtilis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| heme | - |
Bacillus subtilis | |
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