Cloned (Comment) | Organism |
---|---|
C-terminal His8-tagged SfmD is overexpressed in Escherichia coli BL21 (DE3) | Streptomyces lavendulae |
Protein Variants | Comment | Organism |
---|---|---|
H191A | mutant loses ability to hydroxylate 3-methyl-L-tyrosine | Streptomyces lavendulae |
H274A | mutant loses ability to hydroxylate 3-methyl-L-tyrosine | Streptomyces lavendulae |
H313A | mutant loses ability to hydroxylate 3-methyl-L-tyrosine. Heme content of the mutant enzyme is very low, very little heme-Fe binding ability | Streptomyces lavendulae |
H317A | mutant loses ability to hydroxylate 3-methyl-L-tyrosine. Heme content of the mutant enzyme is very low, very little heme-Fe binding ability | Streptomyces lavendulae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.63 | - |
3-methyl-L-tyrosine | pH 9.0, 25°C | Streptomyces lavendulae | |
1 | - |
L-tyrosine | pH 9.0, 25°C | Streptomyces lavendulae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
39800 | - |
x * 39800, SDS-PAGE | Streptomyces lavendulae |
39854 | - |
x * 39854, calculated from sequence (without cofactor) | Streptomyces lavendulae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-methyl-L-tyrosine + H2O2 + H+ | Streptomyces lavendulae | the enzyme is involved in biosynthesis of saframycin A, a potent antitumor antibiotic that belongs to the tetrahydroisoquinoline family | 3-hydroxy-5-methyl-L-tyrosine + H2O | - |
? | |
3-methyl-L-tyrosine + H2O2 + H+ | Streptomyces lavendulae NRRL 11002 | the enzyme is involved in biosynthesis of saframycin A, a potent antitumor antibiotic that belongs to the tetrahydroisoquinoline family | 3-hydroxy-5-methyl-L-tyrosine + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces lavendulae | B0CN28 | - |
- |
Streptomyces lavendulae NRRL 11002 | B0CN28 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Streptomyces lavendulae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-methyl-L-tyrosine + H2O2 + H+ | the enzyme is involved in biosynthesis of saframycin A, a potent antitumor antibiotic that belongs to the tetrahydroisoquinoline family | Streptomyces lavendulae | 3-hydroxy-5-methyl-L-tyrosine + H2O | - |
? | |
3-methyl-L-tyrosine + H2O2 + H+ | the enzyme catalyzes the regioselective hydroxylation of 3-methyltyrosine to 3-hydroxy-5-methyl-L-tyrosine | Streptomyces lavendulae | 3-hydroxy-5-methyl-L-tyrosine + H2O | - |
? | |
3-methyl-L-tyrosine + H2O2 + H+ | the enzyme is involved in biosynthesis of saframycin A, a potent antitumor antibiotic that belongs to the tetrahydroisoquinoline family | Streptomyces lavendulae NRRL 11002 | 3-hydroxy-5-methyl-L-tyrosine + H2O | - |
? | |
3-methyl-L-tyrosine + H2O2 + H+ | the enzyme catalyzes the regioselective hydroxylation of 3-methyltyrosine to 3-hydroxy-5-methyl-L-tyrosine | Streptomyces lavendulae NRRL 11002 | 3-hydroxy-5-methyl-L-tyrosine + H2O | - |
? | |
L-tyrosine + H2O2 + H+ | - |
Streptomyces lavendulae | ? | - |
? | |
L-tyrosine + H2O2 + H+ | - |
Streptomyces lavendulae NRRL 11002 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 39800, SDS-PAGE | Streptomyces lavendulae |
? | x * 39854, calculated from sequence (without cofactor) | Streptomyces lavendulae |
Synonyms | Comment | Organism |
---|---|---|
3-methyl-L-tyrosine peroxidase | - |
Streptomyces lavendulae |
3-methyltyrosine peroxidase | - |
Streptomyces lavendulae |
SacD | - |
Streptomyces lavendulae |
SfmD | - |
Streptomyces lavendulae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Streptomyces lavendulae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.205 | - |
L-tyrosine | pH 9.0, 25°C | Streptomyces lavendulae | |
0.476 | - |
3-methyl-L-tyrosine | pH 9.0, 25°C | Streptomyces lavendulae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Streptomyces lavendulae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | a heme containing protein. The conserved motif HXXXC is crucial for heme binding. SfmD binds one molecular of heme | Streptomyces lavendulae |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme is involved in biosynthesis of saframycin A, a potent antitumor antibiotic that belongs to the tetrahydroisoquinoline family | Streptomyces lavendulae |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.205 | - |
L-tyrosine | pH 9.0, 25°C | Streptomyces lavendulae | |
0.76 | - |
3-methyl-L-tyrosine | pH 9.0, 25°C | Streptomyces lavendulae |