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Literature summary for 1.11.2.6 extracted from
- A heme peroxidase with a functional role as an L-tyrosine hydroxylase in the biosynthesis of anthramycin (2011), Biochemistry, 50, 8926-8936 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Streptomyces refuineus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| H2O2 | the enzyme becomes inactivated by hydrogen peroxide during catalysis | Streptomyces refuineus |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.45 | - |
L-tyrosine | at pH 8.0 and 37°C | Streptomyces refuineus | |
| 1 | - |
H2O2 | with L-tyrosine as cosubstrate, at pH 8.0 and 37°C | Streptomyces refuineus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-tyrosine + H2O2 | Streptomyces refuineus | - |
L-dopa + H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces refuineus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-Sepharose column chromatography or Q-Sepharose column chromatography and S-200HR gel filtration | Streptomyces refuineus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-(4-hydroxylphenyl)propanoic acid + H2O2 | - |
Streptomyces refuineus | 3-(3,4-hydroxyphenyl)propanoic acid + H2O | - |
? | |
| 4-cresol + H2O2 | - |
Streptomyces refuineus | 4-methylcatechol + H2O | - |
? | |
| DL-tyrosine + H2O2 | - |
Streptomyces refuineus | L-DOPA + H2O | - |
? | |
| L-tyrosine + H2O2 | - |
Streptomyces refuineus | L-dopa + H2O | - |
? | |
| additional information | no activity with L-phenylalanine | Streptomyces refuineus | ? | - |
- |
|
| tyramine + H2O2 | - |
Streptomyces refuineus | dopamine + H2O | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 33600, SDS-PAGE | Streptomyces refuineus |
| monomer | 1 * 37900, calculated from amino acid sequence | Streptomyces refuineus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Orf13 | - |
Streptomyces refuineus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.576 | - |
L-tyrosine | at pH 8.0 and 37°C | Streptomyces refuineus | |
| 1.5 | - |
H2O2 | with L-tyrosine as cosubstrate, at pH 8.0 and 37°C | Streptomyces refuineus | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| heme b | required for catalysis | Streptomyces refuineus | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.012 | - |
L-tyrosine | at pH 8.0 and 37°C | Streptomyces refuineus | |
| 0.015 | - |
H2O2 | with L-tyrosine as cosubstrate, at pH 8.0 and 37°C | Streptomyces refuineus | |
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