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Literature summary for extracted from

  • Schneider, K.; Cammack, R.; Schlegel, H.G.; Hall, D.O.
    The iron-sulphur centres of soluble hydrogenase from Alcaligenes eutrophus (1979), Biochim. Biophys. Acta, 578, 445-461.
    View publication on PubMed


Inhibitors Comment Organism Structure
CdSO4 1 mM, complete inhibition Cupriavidus necator
CuCl2 0.2 mM, complete inhibition Cupriavidus necator
HgCl2 0.001 mM, complete inhibition Cupriavidus necator
iodoacetate 1 mM, 10% inhibition Cupriavidus necator
additional information not inhibited by O2, CO and chelating agents Cupriavidus necator
p-chloromercuribenzoate 0.15 mM, complete inhibition Cupriavidus necator


Localization Comment Organism GeneOntology No. Textmining
Cupriavidus necator


Metals/Ions Comment Organism Structure
Iron 11.5 iron atoms per enzyme molecule, enzyme contains 2 [4Fe-4S] and 2 [2Fe-2S] clusters Cupriavidus necator


Organism UniProt Comment Textmining
Cupriavidus necator
formerly Alcaligenes eutrophus

Purification (Commentary)

Purification (Comment) Organism
Cupriavidus necator

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
Cupriavidus necator

Storage Stability

Storage Stability Organism
-20°C, 4 mg/ml protein concentration, pH 7.0 Cupriavidus necator

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
H2 + NAD+
Cupriavidus necator H+ + NADH