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Literature summary for 1.12.1.2 extracted from

  • Leul, M.; Mohapatra, A.; Sellstedt, A.
    Biodiversity of hydrogenases in Frankia (2005), Curr. Microbiol., 50, 17-23.
    View publication on PubMed
Search for more literature for 1.12.1.2

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
H2 + NAD+ Frankia sp. physiological function of the enzyme in Frankia strains, overview H+ + NADH
-
 r

Organism

Organism UniProt Comment Textmining
Frankia sp.
-
 isolates of nine different actinorhizal host plants, 18 strains, overview
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 no H2 producing activity in several strains grown without nitrogen and nickel, overview Frankia sp.
0.035
-
 H2 producing activity, strain R43 grown without nitrogen and nickel, from host plant Compotonia cunninghamiana, with reduced methyl viologen as electron donor Frankia sp.
0.11
-
 H2 producing activity, strain UGL011101 grown without nitrogen and nickel, from host plant Alnus incana, with reduced methyl viologen as electron donor Frankia sp.
0.3
-
 H2 producing activity, strain HFPCcI3 grown without nitrogen and nickel, from host plant Compotonia cunninghamiana, with reduced methyl viologen as electron donor Frankia sp.
4.91
-
 H2 producing activity, strain UGL140102 grown without nitrogen and nickel, from host plant Hippophae rhamnoides, with reduced methyl viologen as electron donor Frankia sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
H2 + NAD+
-
 Frankia sp. H+ + NADH
-
 r
H2 + NAD+ physiological function of the enzyme in Frankia strains, overview Frankia sp. H+ + NADH
-
 r
H2 + oxidized methyl viologen
-
 Frankia sp. H+ + reduced methyl viologen
-
 r

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
27
-
 assay at Frankia sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Frankia sp.

Cofactor

Cofactor Comment Organism Structure
additional information methyl viologen, oxidized and reduced can replace NAD+/NADH Frankia sp.
NAD+
-
 Frankia sp.
NADH
-
 Frankia sp.