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show all sequences of 1.12.1.5

Characterization of hydrogenase II from the hyperthermophilic archaeon Pyrococcus furiosus and assessment of its role in sulfur reduction

Ma, K.; Weiss, R.; Adams, M.W.W.; J. Bacteriol. 182, 1864-1871 (2000)

Data extracted from this reference:

Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytoplasm
-
Pyrococcus furiosus
5737
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Fe2+
contains 21 atoms iron per mol enzyme
Pyrococcus furiosus
Ni2+
contains 0.9 atoms nickel per mol enzyme
Pyrococcus furiosus
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
24000
-
1 * 52000 + 1 * 39000 + 1 * 30000 + 1 * 24000, SDS-PAGE
Pyrococcus furiosus
30000
-
1 * 52000 + 1 * 39000 + 1 * 30000 + 1 * 24000, SDS-PAGE
Pyrococcus furiosus
39000
-
1 * 52000 + 1 * 39000 + 1 * 30000 + 1 * 24000, SDS-PAGE
Pyrococcus furiosus
52000
-
1 * 52000 + 1 * 39000 + 1 * 30000 + 1 * 24000, SDS-PAGE
Pyrococcus furiosus
320000
-
gel filtration
Pyrococcus furiosus
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pyrococcus furiosus
-
-
-
Pyrococcus furiosus
E7FHC4
subunit alpha
-
Pyrococcus furiosus
E7FHF8
subunit delta
-
Pyrococcus furiosus
E7FHN9
subunit beta
-
Pyrococcus furiosus
E7FHW8
subunit gamma
-
Purification (Commentary)
Commentary
Organism
DEAE column chromatography, hydroxyapatite column chromatography, phenyl Sepharose column chromatography, and Superdex 200 gel filtration
Pyrococcus furiosus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
H2 + NAD+
-
439641
Pyrococcus furiosus
H+ + NADH
-
-
-
r
H2 + NADP+
-
439641
Pyrococcus furiosus
H+ + NADPH
-
-
-
r
additional information
ferredoxin from Pyrococcus furiosus is not an efficient electron carrier
439641
Pyrococcus furiosus
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
heterotetramer
1 * 52000 + 1 * 39000 + 1 * 30000 + 1 * 24000, SDS-PAGE
Pyrococcus furiosus
Cofactor
Cofactor
Commentary
Organism
Structure
FAD
contains 0.83 FAD per mol enzyme
Pyrococcus furiosus
NADPH
-
Pyrococcus furiosus
[2Fe-2S]-center
contains 1 [2Fe-2S]-center
Pyrococcus furiosus
[4Fe-4S]-center
contains 2 [4Fe-4S]-centers
Pyrococcus furiosus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
FAD
contains 0.83 FAD per mol enzyme
Pyrococcus furiosus
NADPH
-
Pyrococcus furiosus
[2Fe-2S]-center
contains 1 [2Fe-2S]-center
Pyrococcus furiosus
[4Fe-4S]-center
contains 2 [4Fe-4S]-centers
Pyrococcus furiosus
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytoplasm
-
Pyrococcus furiosus
5737
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Fe2+
contains 21 atoms iron per mol enzyme
Pyrococcus furiosus
Ni2+
contains 0.9 atoms nickel per mol enzyme
Pyrococcus furiosus
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
24000
-
1 * 52000 + 1 * 39000 + 1 * 30000 + 1 * 24000, SDS-PAGE
Pyrococcus furiosus
30000
-
1 * 52000 + 1 * 39000 + 1 * 30000 + 1 * 24000, SDS-PAGE
Pyrococcus furiosus
39000
-
1 * 52000 + 1 * 39000 + 1 * 30000 + 1 * 24000, SDS-PAGE
Pyrococcus furiosus
52000
-
1 * 52000 + 1 * 39000 + 1 * 30000 + 1 * 24000, SDS-PAGE
Pyrococcus furiosus
320000
-
gel filtration
Pyrococcus furiosus
Purification (Commentary) (protein specific)
Commentary
Organism
DEAE column chromatography, hydroxyapatite column chromatography, phenyl Sepharose column chromatography, and Superdex 200 gel filtration
Pyrococcus furiosus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
H2 + NAD+
-
439641
Pyrococcus furiosus
H+ + NADH
-
-
-
r
H2 + NADP+
-
439641
Pyrococcus furiosus
H+ + NADPH
-
-
-
r
additional information
ferredoxin from Pyrococcus furiosus is not an efficient electron carrier
439641
Pyrococcus furiosus
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
heterotetramer
1 * 52000 + 1 * 39000 + 1 * 30000 + 1 * 24000, SDS-PAGE
Pyrococcus furiosus
Other publictions for EC 1.12.1.5
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
722777
Chandrayan
Engineering hyperthermophilic ...
Pyrococcus furiosus, Pyrococcus furiosus COM1
J. Biol. Chem.
287
3257-3264
2012
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1
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1
1
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3
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1
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3
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4
1
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1
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4
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1
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4
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-
-
-
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1
1
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1
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3
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4
1
-
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1
-
-
-
-
-
-
-
-
-
-
-
439641
Ma
Characterization of hydrogenas ...
Pyrococcus furiosus
J. Bacteriol.
182
1864-1871
2000
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-
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1
2
5
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5
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1
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3
1
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-
-
-
-
-
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4
-
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-
-
-
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4
-
-
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-
-
-
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1
2
5
-
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1
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3
1
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