Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CO | - |
Desulfovibrio desulfuricans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.075 | - |
ferrocytochrome c3 | H2-evolution reaction | Desulfovibrio desulfuricans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60000 | - |
gel filtration | Desulfovibrio desulfuricans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Desulfovibrio desulfuricans | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Desulfovibrio desulfuricans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
H+ + reduced methyl viologen | - |
Desulfovibrio desulfuricans | H2 + methyl viologen | - |
? | |
H2 + ferricytochrome c3 | - |
Desulfovibrio desulfuricans | H+ + ferrocytochrome c3 | - |
r | |
additional information | enzyme catalyzes production of H2 from Na2S2O4 in presence of cytochrome c3 | Desulfovibrio desulfuricans | ? | - |
? | |
additional information | enzyme can catalyze H-2H exchange in absence of added electron carriers | Desulfovibrio desulfuricans | ? | - |
? | |
additional information | no reduction of ferredoxin, methylene blue or hexacyanoferrate(II) by H2 | Desulfovibrio desulfuricans | ? | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
10 min, H2 evolution in presence of methyl viologen, no decrease in activity | Desulfovibrio desulfuricans |
60 | - |
10 min, H2 evolution in presence of methyl viologen, 30% loss of activity | Desulfovibrio desulfuricans |
70 | - |
10 min, H2 evolution in presence of methyl viologen, 37% loss of activity | Desulfovibrio desulfuricans |
80 | - |
10 min, H2 evolution in presence of methyl viologen, 69% loss of activity | Desulfovibrio desulfuricans |
90 | - |
10 min, H2 evolution in presence of methyl viologen, 99% loss of activity | Desulfovibrio desulfuricans |
100 | - |
10 min, H2 evolution in presence of methyl viologen, complete loss of activity | Desulfovibrio desulfuricans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | 6 | cytochrome c3-dependent H2 evolution | Desulfovibrio desulfuricans |
6 | 7 | methyl viologen-dependent H2 evolution | Desulfovibrio desulfuricans |
6 | 8 | H-2H exchange | Desulfovibrio desulfuricans |
8 | 9 | cytochrome c3 reduction by H2 | Desulfovibrio desulfuricans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
cytochrome c3 | - |
Desulfovibrio desulfuricans |