Literature summary for 1.12.2.1 extracted from

Yagi, T.; Kimura, K.; Daidoji, H.; Sakai, F.; Tamura, S.; Inokuchi, H.
Properties of purified hydrogenase from the particulate fraction of Desulfovibrio vulgaris, Miyazaki (1976), J. Biochem., 79, 661-671.

Search for more literature for 1.12.2.1

Inhibitors

Inhibitors	Comment	Organism	Structure
CO	-	Desulfovibrio vulgaris
EDTA	10 mM, 10% inhibition	Desulfovibrio vulgaris
H2O2	-	Desulfovibrio vulgaris
NEM	2 mM; 60% inhibition	Desulfovibrio vulgaris
p-chloromercuribenzene sulfonate	1 mM; 87% inhibition	Desulfovibrio vulgaris
PCMB	0.17 mM, 35% inhibition	Desulfovibrio vulgaris
SDS	0.1%, 86-99% inactivation	Desulfovibrio vulgaris

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
particle-bound	-	Desulfovibrio vulgaris	-	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Iron	iron-sulfur protein, 7-9 iron atoms and 7-8 labile sulfide ions	Desulfovibrio vulgaris

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
28000	-	1 * 28000 + 1 * 59000, SDS-PAGE	Desulfovibrio vulgaris
59000	-	1 * 28000 + 1 * 59000, SDS-PAGE	Desulfovibrio vulgaris
89000	-	low-speed equilibrium sedimentation	Desulfovibrio vulgaris

Organic Solvent Stability

Organic Solvent	Comment	Organism
urea	9 M, 24 h, room temperature, stable	Desulfovibrio vulgaris

Organism

Organism	UniProt	Comment	Textmining
Desulfovibrio vulgaris	-	Miyazaki	-
Desulfovibrio vulgaris Miyazaki	-	Miyazaki	-

Purification (Commentary)

Purification (Comment)	Organism
-	Desulfovibrio vulgaris

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
H+ + reduced methyl viologen	-	Desulfovibrio vulgaris	H2 + methyl viologen	-	?
H+ + reduced methyl viologen	-	Desulfovibrio vulgaris Miyazaki	H2 + methyl viologen	-	?
H2 + ferricytochrome c3	-	Desulfovibrio vulgaris	H+ + ferrocytochrome c3	-	?
H2 + ferricytochrome c3	-	Desulfovibrio vulgaris Miyazaki	H+ + ferrocytochrome c3	-	?

Subunits

Subunits	Comment	Organism
dimer	1 * 28000 + 1 * 59000, SDS-PAGE	Desulfovibrio vulgaris

Cofactor

Cofactor	Comment	Organism	Structure
cytochrome c3	-	Desulfovibrio vulgaris

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
additional information	-	additional information	Ki for CO is 8.9 Torr	Desulfovibrio vulgaris

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Release 2023.1 (January 2023)