Inhibitors | Comment | Organism | Structure |
---|---|---|---|
O2 | reversible inhibition | Pyrococcus furiosus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe | 31 atoms per mol | Pyrococcus furiosus | |
Ni | 0.98 atoms per mol enzyme | Pyrococcus furiosus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
24000 | - |
alpha, beta, gamma, 2 * 46000 + 2 * 27000 + 2 * 24000, SDS-PAGE | Pyrococcus furiosus |
27000 | - |
alpha, beta, gamma, 2 * 46000 + 2 * 27000 + 2 * 24000, SDS-PAGE | Pyrococcus furiosus |
46000 | - |
alpha, beta, gamma, 2 * 46000 + 2 * 27000 + 2 * 24000, SDS-PAGE | Pyrococcus furiosus |
185000 | - |
gel filtration | Pyrococcus furiosus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
H2 + oxidized ferredoxin | Pyrococcus furiosus | - |
H+ + reduced ferredoxin | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus furiosus | - |
strain DSM 3638 | - |
Purification (Comment) | Organism |
---|---|
- |
Pyrococcus furiosus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
H2 + electron acceptor | methylene blue as electron acceptor | Pyrococcus furiosus | H+ + reduced electron acceptor | - |
r | |
H2 + electron acceptor | methyl viologen as electron acceptor | Pyrococcus furiosus | H+ + reduced electron acceptor | - |
r | |
H2 + oxidized ferredoxin | - |
Pyrococcus furiosus | H+ + reduced ferredoxin | - |
r |
Subunits | Comment | Organism |
---|---|---|
hexamer | alpha, beta, gamma, 2 * 46000 + 2 * 27000 + 2 * 24000, SDS-PAGE | Pyrococcus furiosus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
95 | - |
hydrogen evolution | Pyrococcus furiosus |
95 | - |
above 95°C, hydrogen oxidation | Pyrococcus furiosus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
100 | - |
most of activity is retained after 1 h incubation | Pyrococcus furiosus |