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Literature summary for 1.12.98.1 extracted from
- FAD requirement for the reduction of coenzyme F420 by hydrogenase from Methanobacterium formicicum (1984), Biochem. Biophys. Res. Commun., 120, 775-781.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| ammonium sulfate | improves reactivation of enzyme with FAD after FAD depletion | Methanobacterium formicicum |  |
| FAD | restores activity after depletion of FAD in hydrophobic interaction chromatography | Methanobacterium formicicum | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Iron | - |
Methanobacterium formicicum | |
| Nickel | - |
Methanobacterium formicicum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 170000 | - |
gel filtration | Methanobacterium formicicum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| H2 + coenzyme F420 | Methanobacterium formicicum | enzyme of methanogenesis pathway | reduced coenzyme F420 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Methanobacterium formicicum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Methanobacterium formicicum |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.24 | - |
F420-linked activity | Methanobacterium formicicum |
| 63.2 | - |
methyl viologen-linked activity | Methanobacterium formicicum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| H2 + coenzyme F420 | - |
Methanobacterium formicicum | reduced coenzyme F420 | - |
? | |
| H2 + coenzyme F420 | enzyme of methanogenesis pathway | Methanobacterium formicicum | reduced coenzyme F420 | - |
? | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | bound to protein, depleted by hydrophobic interaction chromatography | Methanobacterium formicicum | |
| additional information | no activity with FMN after FAD depletion | Methanobacterium formicicum |
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