Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | conditions for anaerobic reductive activation in the presence of hydrogen, 2-mercaptoethanol and KCl or methyl viologen yield maximal hydrogenase activity | Methanosarcina barkeri |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0033 | - |
oxidized methyl viologen | 37°C, pH 8.3 | Methanosarcina barkeri | |
0.025 | - |
oxidized coenzyme F420 | 37°C, pH 7.5 | Methanosarcina barkeri |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
KCl | increases activity, maximal activity at 250 mM | Methanosarcina barkeri |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
8000 | - |
x * 8000 + x * 33000 + x * 30000, SDS-PAGE | Methanosarcina barkeri |
30000 | - |
x * 8000 + x * 33000 + x * 30000, SDS-PAGE | Methanosarcina barkeri |
33000 | - |
x * 8000 + x * 33000 + x * 30000, SDS-PAGE | Methanosarcina barkeri |
198000 | - |
non-denaturing PAGE, small enzyme form | Methanosarcina barkeri |
845000 | - |
non-denaturing PAGE, large enzyme form | Methanosarcina barkeri |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
H2 + oxidized coenzyme F420 | Methanosarcina barkeri | - |
reduced coenzyme F420 | - |
? | |
H2 + oxidized coenzyme F420 | Methanosarcina barkeri DSM 804 | - |
reduced coenzyme F420 | - |
? | |
H2 + oxidized methyl viologen | Methanosarcina barkeri | - |
reduced methyl viologen | - |
? | |
H2 + oxidized methyl viologen | Methanosarcina barkeri DSM 804 | - |
reduced methyl viologen | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanosarcina barkeri | P80490 and P80489 and P80491 | P80490: subunit beta, P80489: subunit alpha, P80491: subunit gamma | - |
Methanosarcina barkeri DSM 804 | P80490 and P80489 and P80491 | P80490: subunit beta, P80489: subunit alpha, P80491: subunit gamma | - |
Oxidation Stability | Organism |
---|---|
enzyme activity is unstable under reducing conditions | Methanosarcina barkeri |
Purification (Comment) | Organism |
---|---|
- |
Methanosarcina barkeri |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
11.5 | - |
37°C, pH 7.5, substrate: oxidized coenzyme F420 | Methanosarcina barkeri |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
H2 + oxidized coenzyme F420 | - |
Methanosarcina barkeri | reduced coenzyme F420 | - |
? | |
H2 + oxidized coenzyme F420 | - |
Methanosarcina barkeri DSM 804 | reduced coenzyme F420 | - |
? | |
H2 + oxidized methyl viologen | - |
Methanosarcina barkeri | reduced methyl viologen | - |
? | |
H2 + oxidized methyl viologen | - |
Methanosarcina barkeri DSM 804 | reduced methyl viologen | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 8000 + x * 33000 + x * 30000, SDS-PAGE | Methanosarcina barkeri |
Synonyms | Comment | Organism |
---|---|---|
F420-reducing hydrogenase | - |
Methanosarcina barkeri |
Mbar_A0449 | subunit beta | Methanosarcina barkeri |
Mbar_A0450 | subunit gamma | Methanosarcina barkeri |
Mbar_A0452 | subunit alpha | Methanosarcina barkeri |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
353 | - |
oxidized coenzyme F420 | 37°C, pH 7.5 | Methanosarcina barkeri | |
9226 | - |
oxidized methyl viologen | 37°C, pH 8.3 | Methanosarcina barkeri |