Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | transmembrane protein | Geobacter metallireducens | 16020 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
H2 + oxidized coenzyme F420 | Geobacter metallireducens | - |
reduced coenzyme F420 | - |
? | |
additional information | Geobacter metallireducens | generation of F420H2 by Fpo with electrons derived from DIET requires an abundance of reduced methanophenazine | ? | - |
- |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacter metallireducens | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
H2 + oxidized coenzyme F420 | - |
Geobacter metallireducens | reduced coenzyme F420 | - |
? | |
additional information | generation of F420H2 by Fpo with electrons derived from DIET requires an abundance of reduced methanophenazine | Geobacter metallireducens | ? | - |
- |
Synonyms | Comment | Organism |
---|---|---|
F420H2 dehydrogenase | - |
Geobacter metallireducens |
Fpo | - |
Geobacter metallireducens |
Organism | Comment | Expression |
---|---|---|
Geobacter metallireducens | transcripts for genes for most of the subunits for the membranebound F420H2 dehydrogenase, Fpo, are higher in direct interspecies electron transfer (DIET)-grown cells compared to hydrogen interspecies electron transfer (HIT) grown cells, overview | up |
General Information | Comment | Organism |
---|---|---|
metabolism | electrons delivered to methanophenazine in the cell membrane are transferred to Fpo. Proton translocation drives Fpo-catalyzed reduction of F420 to F420H2. Half of the F420H2 produced serves as a reductant in the carbon dioxide reduction pathway. The remaining F420H2 is the electron donor for HdrABC, which reduces ferredoxin and CoM-S-S-CoB in an electron bifurcation reaction. Fpo plays a key role in electron transport for carbon dioxide reduction to methane during direct interspecies electron transfer (DIET) | Geobacter metallireducens |
physiological function | the external proton gradient necessary to drive the otherwise thermodynamically unfavorable reverse electron transport for F420H2 dehydrogenase (Fpo)-catalyzed F420 reduction (in Methanosarcina barkeri) is derived from protons released from Geobacter metallireducens metabolism. Enzyme Fpo plays a key role in electron transport for carbon dioxide reduction to methane during direct interspecies electron transfer (DIET). During methylotrophic methanogenesis in Methanosarcina barkeri, Fpo oxidizes F420H2 with the reduction of methanophenazine in the membrane, coupled with vectorial proton translocation to the outside of the membrane. Under some conditions Fpo may catalyze the reverse reaction in which reduced methanophenazine serves as the electron donor for the reduction of F420. In this direction, proton translocation through Fpo into the cytoplasm is required in order to make the reaction thermodynamically favorable. The Fpo-catalyzed reduction of F420 is proton balanced | Geobacter metallireducens |