Cloned (Comment) | Organism |
---|---|
overproduced in Escherichia coli BL21(DE3) cells | Methanocaldococcus jannaschii |
Crystallization (Comment) | Organism |
---|---|
mutant C176A crystallized in the presence of dithiothreitol, at 1.95 A resolution | Methanocaldococcus jannaschii |
Protein Variants | Comment | Organism |
---|---|---|
C176A | the Cys176 sulfur and unknown ligands of the iron complex of the wild-type enzyme are replaced by the dithiothreitol present in the crystallization solution | Methanocaldococcus jannaschii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CO | - |
Methanocaldococcus jannaschii | |
cyanide | - |
Methanocaldococcus jannaschii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe | iron center octahedrally coordinated by one dithiothreitol-sulfur and one dithiothreitol-oxygen, two CO, the nitrogen of 2-pyridinol and the 6-formylmethyl group of 2-pyridinol in an acyliron ligation | Methanocaldococcus jannaschii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanocaldococcus jannaschii | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Methanocaldococcus jannaschii |
Synonyms | Comment | Organism |
---|---|---|
[Fe]-hydrogenase | - |
Methanocaldococcus jannaschii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | iron-guanylylpyridinol | Methanocaldococcus jannaschii |