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Literature summary for 1.12.99.6 extracted from

  • Yoon, K.S.; Sakai, Y.; Tsukada, N.; Fujisawa, K.; Nishihara, H.
    Purification and biochemical characterization of a membrane-bound [NiFe]-hydrogenase from a hydrogen-oxidizing, lithotrophic bacterium, Hydrogenophaga sp. AH-24 (2009), FEMS Microbiol. Lett., 290, 114-120.
    View publication on PubMed
Search for more literature for 1.12.99.6

Activating Compound

Activating Compound Comment Organism Structure
additional information activity increases up to 2.5fold during incubation at 50°C under H2 Hydrogenophaga sp.

Inhibitors

Inhibitors Comment Organism Structure
O2 enzyme is quite stable under air atmosphere, half-life of activity is ca. 48 h at 4°C, inactivation is irreversible Hydrogenophaga sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.025
-
 oxidized methylene blue pH 7.0, 30°C Hydrogenophaga sp.
1.78
-
 benzyl viologen pH 7.0, 30°C Hydrogenophaga sp.
12.5
-
 methyl viologen pH 7.0, 30°C Hydrogenophaga sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Hydrogenophaga sp. 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Fe membrane-bound [NiFe]-hydrogenase exhibits prominent electron paramagnetic resonance signals originating from [3Fe–4S]1 and [4Fe–4S]1 clusters Hydrogenophaga sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37000
-
 1 * 62000 + 1 * 37000, SDS-PAGE Hydrogenophaga sp.
62000
-
 1 * 62000 + 1 * 37000, SDS-PAGE Hydrogenophaga sp.
100000
-
 gel filtration Hydrogenophaga sp.

Organism

Organism UniProt Comment Textmining
Hydrogenophaga sp.
-
 AH-24
-
Hydrogenophaga sp. AH-24
-
 AH-24
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, by gel filtration, 133fold purified and 68.2% of yield relative to the membrane Hydrogenophaga sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
110
-
 purified enzyme Hydrogenophaga sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
H2 + oxidized benzyl viologen
-
 Hydrogenophaga sp. H+ + reduced benzyl viologen
-
 ?
H2 + oxidized benzyl viologen
-
 Hydrogenophaga sp. AH-24 H+ + reduced benzyl viologen
-
 ?
H2 + oxidized methyl viologen
-
 Hydrogenophaga sp. H+ + reduced methyl viologen
-
 ?
H2 + oxidized methyl viologen
-
 Hydrogenophaga sp. AH-24 H+ + reduced methyl viologen
-
 ?
H2 + oxidized methylene blue
-
 Hydrogenophaga sp. H+ + reduced methylene blue
-
 ?
H2 + oxidized methylene blue
-
 Hydrogenophaga sp. AH-24 H+ + reduced methylene blue
-
 ?
H2 + oxidized methylene blue
-
 Hydrogenophaga sp. reduced methylene blue + H+
-
 r
H2 + oxidized methylene blue
-
 Hydrogenophaga sp. AH-24 reduced methylene blue + H+
-
 r
H2 + oxidized methylene blue
-
 Hydrogenophaga sp. reduced methylene blue
-
 r
H2 + oxidized methylene blue
-
 Hydrogenophaga sp. AH-24 reduced methylene blue
-
 r

Subunits

Subunits Comment Organism
heterodimer 1 * 62000 + 1 * 37000, SDS-PAGE Hydrogenophaga sp.

Synonyms

Synonyms Comment Organism
Mbh
-
 Hydrogenophaga sp.
membrane-bound [NiFe]-hydrogenase
-
 Hydrogenophaga sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
75
-
-
 Hydrogenophaga sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 under anaerobic conditions, retains full activity for over 5 h Hydrogenophaga sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
20
-
 methyl viologen pH 7.0, 30°C Hydrogenophaga sp.
240
-
 benzyl viologen pH 7.0, 30°C Hydrogenophaga sp.
560
-
 methylene blue pH 7.0, 30°C Hydrogenophaga sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4
-
 H2 evolution Hydrogenophaga sp.
8
-
 H2 oxidation Hydrogenophaga sp.

pH Range

pH Minimum pH Maximum Comment Organism
3.5 9 H2 evolution, pH 3.5 and 9.0: ca. 50% of maximal activity Hydrogenophaga sp.
5.5 9 H2 oxidation, pH 5.5: 40% of maximal activity, pH 9: 60% of maximal activity Hydrogenophaga sp.

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.6
-
 methyl viologen pH 7.0, 30°C Hydrogenophaga sp.
135
-
 benzyl viologen pH 7.0, 30°C Hydrogenophaga sp.
22400
-
 methylene blue pH 7.0, 30°C Hydrogenophaga sp.