Literature summary for 1.12.99.6 extracted from

Ludwig, M.; Schubert, T.; Zebger, I.; Wisitruangsakul, N.; Saggu, M.; Strack, A.; Lenz, O.; Hildebrandt, P.; Friedrich, B.
Concerted action of two novel auxiliary proteins in assembly of the active site in a membrane-bound [NiFe] hydrogenase (2009), J. Biol. Chem., 284, 2159-2168.

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Activating Compound

Activating Compound	Comment	Organism	Structure
HoxL	the auxiliary protein HoxL participates specifically in the maturation of the large subunit of the enzyme. It is essential for H2-oxidizing activity and enzyme-driven growth on H2. HoxL acts as a specific chaperone assisting the transfer of the Fe(CN-)2CO cofactor intermediate from the Hyp machinery to the enzyme	Cupriavidus necator H16
HoxV	the auxiliary protein HoxL participates specifically in the maturation of the large subunit of the enzyme. It is essential for H2-oxidizing activity and enzyme-driven growth on H2. HoxV functions as a scaffold delivering the Fe(CN-)2CO moiety to the precursor of the large subunit pre-HoxG of the enzyme	Cupriavidus necator H16
HypC	is essential for cofactor assembly	Cupriavidus necator H16

Inhibitors

Inhibitors	Comment	Organism	Structure
additional information	is oxygen-tolerant	Cupriavidus necator H16

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
membrane	-	Cupriavidus necator H16	16020	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
CN-	the auxiliary proteins HoxL and HoxV assist in assembly of the Fe(CN-)2CO moiety	Cupriavidus necator H16
CO	the auxiliary proteins HoxL and HoxV assist in assembly of the Fe(CN-)2CO moiety	Cupriavidus necator H16
Fe	the auxiliary proteins HoxL and HoxV assist in assembly of the Fe(CN-)2CO moiety	Cupriavidus necator H16
Ni2+	Ni-Fe active site assembly, nickel is absent in samples of HoxV	Cupriavidus necator H16

Organism

Organism	UniProt	Comment	Textmining
Cupriavidus necator H16	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
H2 + oxidized methylene blue	-	Cupriavidus necator H16	H+ + reduced methylene blue	-	?

Synonyms

Synonyms	Comment	Organism
Mbh	-	Cupriavidus necator H16
[NiFe] hydrogenase	-	Cupriavidus necator H16

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Release 2023.1 (January 2023)