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Literature summary for 1.12.99.6 extracted from

  • Cracknell, J.A.; Wait, A.F.; Lenz, O.; Friedrich, B.; Armstrong, F.A.
    A kinetic and thermodynamic understanding of O2 tolerance in [NiFe]-hydrogenases (2009), Proc. Natl. Acad. Sci. USA, 106, 20681-20686.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.12.99.6

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0004
-
 H2 at pH 5.5, 10°C Cupriavidus necator
0.001
-
 H2 at pH 5.5, 20°C Cupriavidus necator
0.006
-
 H2 at pH 4.5, 30°C Cupriavidus necator
0.006
-
 H2 at pH 5.5, 30°C Cupriavidus necator
0.008
-
 H2 at pH 5.0, 30°C Cupriavidus necator
0.01
-
 H2 at pH 6.5, 30°C Cupriavidus necator
0.015
-
 H2 at pH 6.0, 30°C Cupriavidus necator
0.024
-
 H2 at pH 5.5, 40°C Cupriavidus necator

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Cupriavidus necator 16020
-

Organism

Organism UniProt Comment Textmining
Cupriavidus necator
-
-
-
Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1
-
-
-

Oxidation Stability

Oxidation Stability Organism
MBH is an O2-tolerant hydrogenase, O2 tolerance increases with increasing temperature (10-40°C) and decreasing potentials (from -0.018 mV to +0.262 mV) Cupriavidus necator

Purification (Commentary)

Purification (Comment) Organism
-
 Cupriavidus necator

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
H2 + O2
-
 Cupriavidus necator ?
-
 ?
H2 + O2
-
 Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 ?
-
 ?

Synonyms

Synonyms Comment Organism
Mbh
-
 Cupriavidus necator
uptake [NiFe]-hydrogenase
-
 Cupriavidus necator
[NiFe]-hydrogenase
-
 Cupriavidus necator