Literature summary for 1.12.99.6 extracted from

Flanagan, L.A.; Wright, J.J.; Roessler, M.M.; Moir, J.W.; Parkin, A.
Re-engineering a NiFe hydrogenase to increase the H2 production bias while maintaining native levels of O2 tolerance (2016), Chem. Commun. (Camb.), 52, 9133-9136 .

Search for more literature for 1.12.99.6

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli	Escherichia coli

Protein Variants

Protein Variants	Comment	Organism
E73A	the catalytic activity of the mutant is comparable to native enzyme	Escherichia coli

Inhibitors

Inhibitors	Comment	Organism	Structure
O2	-	Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
H2 + methylene blue	Escherichia coli	-	reduced methylene blue	-	?

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
HisTrap Ni affinity column chromatography, and Superdex 200 gel filtration	Escherichia coli

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
H2 + methylene blue	-	Escherichia coli	reduced methylene blue	-	?

Synonyms

Synonyms	Comment	Organism
Hyd-1	-	Escherichia coli
hydrogenase-1	-	Escherichia coli
NiFe hydrogenase	-	Escherichia coli

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Release 2023.1 (January 2023)