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Literature summary for 1.13.11.1 extracted from

  • Gou, M.; Qu, Y.; Zhou, J.; Li, A.; Salah Uddin, M.
    Characterization of catechol 1,2-dioxygenase from cell extracts of Sphingomonas xenophaga QYY (2009), Sci. China, Ser. B, Chem. Life Sci. Earth Sci., 52, 615-620.
No PubMed abstract available
Search for more literature for 1.13.11.1

Activating Compound

Activating Compound Comment Organism Structure
additional information inducible enzyme, strain QYY synthesize C12O only when it grow on culture in the presence of catechol Sphingobium xenophagum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.05285
-
 catechol
-
 Sphingobium xenophagum

Metals/Ions

Metals/Ions Comment Organism Structure
Ba2+ 1 mmol/l, 30°C, 15 min, 106% remaining activity Sphingobium xenophagum
Ca2+ 1 mmol/l, 30°C, 15 min, 168% remaining activity Sphingobium xenophagum
Co2+ 1 mmol/l, 30°C, 15 min, 52.7% remaining activity Sphingobium xenophagum
Cu2+ 1 mmol/l, 30°C, 15 min, completely inactivated Sphingobium xenophagum
Fe2+ 1 mmol/l, 30°C, 15 min, completely inactivated Sphingobium xenophagum
Fe3+ 1 mmol/l, 30°C, 15 min, ferric-dependent enzyme, 140% remaining activity Sphingobium xenophagum
Hg2+ 1 mmol/l, 30°C, 15 min, 76.2% remaining activity Sphingobium xenophagum
K+ 1 mmol/l, 30°C, 15 min, 1317% remaining activity Sphingobium xenophagum
Mg2+ 1 mmol/l, 30°C, 15 min, 190.7% remaining activity Sphingobium xenophagum
Mn2+ 1 mmol/l, 30°C, 15 min, 89.7% remaining activity Sphingobium xenophagum
Ni2+ 1 mmol/l, 30°C, 15 min, 74.6% remaining activity Sphingobium xenophagum
Pb2+ 1 mmol/l, 30°C, 15 min, 281% remaining activity Sphingobium xenophagum
Zn2+ 1 mmol/l, 30°C, 15 min, 2% remaining activity Sphingobium xenophagum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
 SDS-PAGE, value about Sphingobium xenophagum

Organism

Organism UniProt Comment Textmining
Sphingobium xenophagum
-
-
-
Sphingobium xenophagum QYY
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial purification with HiTrap Q Sepharose column chromatography Sphingobium xenophagum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.02
-
 growth condition: LB medium added with 500mg/l catechol Sphingobium xenophagum
0.5
-
 growth condition: catechol Sphingobium xenophagum
1.14
-
 growth condition: tryptone medium added with 500 mg/l catechol Sphingobium xenophagum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
catechol + ?
-
 Sphingobium xenophagum ?
-
 ?
catechol + ?
-
 Sphingobium xenophagum QYY ?
-
 ?

Synonyms

Synonyms Comment Organism
C12O
-
 Sphingobium xenophagum
catechol 1,2-dioxygenase
-
 Sphingobium xenophagum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40 70
-
 Sphingobium xenophagum

pH Range

pH Minimum pH Maximum Comment Organism
7.8 8.8 increase of enzyme activity at pH 5.8-8.8, 29.3% decrease of enzyme activity at pH 9.4, 50% decrease of enzyme activity at pH 10.7 Sphingobium xenophagum