Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Acinetobacter radioresistens |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method, using 2 M ammonium sulfate, 0.1 M Tris-HCl, pH 8.5, at 4°C | Acinetobacter radioresistens |
Protein Variants | Comment | Organism |
---|---|---|
A72G | the mutant shows decreased activity with catechol and no activity with 3-methylcatechol compared to the wild type enzyme | Acinetobacter radioresistens |
L69A | the mutant shows decreased activity with catechol and increased activity for 4-chlorocatechol compared to the wild type enzyme | Acinetobacter radioresistens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0001 | - |
4-Chlorocatechol | mutant enzyme L69A, in 100 mM Tris-HCl pH 8.5, temperature not specified in the publication | Acinetobacter radioresistens | |
0.00198 | - |
4-Chlorocatechol | wild type enzyme, in 100 mM Tris-HCl pH 8.5, temperature not specified in the publication | Acinetobacter radioresistens | |
0.00204 | - |
catechol | wild type enzyme, in 100 mM Tris-HCl pH 8.5, temperature not specified in the publication | Acinetobacter radioresistens | |
0.00225 | - |
3-methylcatechol | wild type enzyme, in 100 mM Tris-HCl pH 8.5, temperature not specified in the publication | Acinetobacter radioresistens | |
0.00576 | - |
catechol | mutant enzyme L69A, in 100 mM Tris-HCl pH 8.5, temperature not specified in the publication | Acinetobacter radioresistens | |
0.00595 | - |
3-methylcatechol | mutant enzyme L69A, in 100 mM Tris-HCl pH 8.5, temperature not specified in the publication | Acinetobacter radioresistens | |
0.0061 | - |
4-Chlorocatechol | mutant enzyme A72G, in 100 mM Tris-HCl pH 8.5, temperature not specified in the publication | Acinetobacter radioresistens | |
0.0093 | - |
catechol | mutant enzyme A72G, in 100 mM Tris-HCl pH 8.5, temperature not specified in the publication | Acinetobacter radioresistens | |
0.01 | - |
4-methylcatechol | mutant enzyme A72G, in 100 mM Tris-HCl pH 8.5, temperature not specified in the publication | Acinetobacter radioresistens | |
0.0128 | - |
4-methylcatechol | mutant enzyme L69A, in 100 mM Tris-HCl pH 8.5, temperature not specified in the publication | Acinetobacter radioresistens | |
0.053 | - |
4-methylcatechol | wild type enzyme, in 100 mM Tris-HCl pH 8.5, temperature not specified in the publication | Acinetobacter radioresistens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe3+ | Fe3+-dependent enzyme | Acinetobacter radioresistens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acinetobacter radioresistens | Q9F103 | - |
- |
Acinetobacter radioresistens LMG S13 | Q9F103 | - |
- |
Purification (Comment) | Organism |
---|---|
Q-Sepharose column chromatography, gel filtration | Acinetobacter radioresistens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-methylcatechol + O2 | the enzyme preferentially recognizes 4-substituted over 3-substituted catechols | Acinetobacter radioresistens | (2Z,4Z)-2-methylhexa-2,4-dienedioate | - |
? | |
3-methylcatechol + O2 | the enzyme preferentially recognizes 4-substituted over 3-substituted catechols | Acinetobacter radioresistens LMG S13 | (2Z,4Z)-2-methylhexa-2,4-dienedioate | - |
? | |
4,5-dichlorocatechol + O2 | the enzyme possesses only a quite rare capability to process 4,5-dichlorocatechol | Acinetobacter radioresistens | ? | - |
? | |
4,5-dichlorocatechol + O2 | the enzyme possesses only a quite rare capability to process 4,5-dichlorocatechol | Acinetobacter radioresistens LMG S13 | ? | - |
? | |
4-chlorocatechol + O2 | the enzyme preferentially recognizes 4-substituted over 3-substituted catechols | Acinetobacter radioresistens | (2E,4Z)-3-chlorohexa-2,4-dienedioate | - |
? | |
4-chlorocatechol + O2 | the enzyme preferentially recognizes 4-substituted over 3-substituted catechols | Acinetobacter radioresistens LMG S13 | (2E,4Z)-3-chlorohexa-2,4-dienedioate | - |
? | |
4-methylcatechol + O2 | the enzyme preferentially recognizes 4-substituted over 3-substituted catechols | Acinetobacter radioresistens | 4-methyl-cis,cis-muconate | - |
? | |
4-methylcatechol + O2 | the enzyme preferentially recognizes 4-substituted over 3-substituted catechols | Acinetobacter radioresistens LMG S13 | 4-methyl-cis,cis-muconate | - |
? | |
catechol + O2 | best substrate | Acinetobacter radioresistens | cis,cis-muconate | - |
? | |
catechol + O2 | best substrate | Acinetobacter radioresistens LMG S13 | cis,cis-muconate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
1,2 CTD | - |
Acinetobacter radioresistens |
catechol 1,2 dioxygenase | - |
Acinetobacter radioresistens |
IsoB | - |
Acinetobacter radioresistens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.17 | - |
4,5-dichlorocatechol | mutant enzyme A72G, in 100 mM Tris-HCl pH 8.5, temperature not specified in the publication | Acinetobacter radioresistens | |
0.17 | - |
4,5-dichlorocatechol | mutant enzyme L69A, in 100 mM Tris-HCl pH 8.5, temperature not specified in the publication | Acinetobacter radioresistens | |
0.18 | - |
4,5-dichlorocatechol | wild type enzyme, in 100 mM Tris-HCl pH 8.5, temperature not specified in the publication | Acinetobacter radioresistens | |
0.34 | - |
4-Chlorocatechol | mutant enzyme L69A, in 100 mM Tris-HCl pH 8.5, temperature not specified in the publication | Acinetobacter radioresistens | |
0.52 | - |
3-methylcatechol | mutant enzyme L69A, in 100 mM Tris-HCl pH 8.5, temperature not specified in the publication | Acinetobacter radioresistens | |
0.72 | - |
4-methylcatechol | mutant enzyme L69A, in 100 mM Tris-HCl pH 8.5, temperature not specified in the publication | Acinetobacter radioresistens | |
0.83 | - |
4-Chlorocatechol | wild type enzyme, in 100 mM Tris-HCl pH 8.5, temperature not specified in the publication | Acinetobacter radioresistens | |
1.59 | - |
3-methylcatechol | wild type enzyme, in 100 mM Tris-HCl pH 8.5, temperature not specified in the publication | Acinetobacter radioresistens | |
1.74 | - |
4-Chlorocatechol | mutant enzyme A72G, in 100 mM Tris-HCl pH 8.5, temperature not specified in the publication | Acinetobacter radioresistens | |
4.42 | - |
catechol | mutant enzyme L69A, in 100 mM Tris-HCl pH 8.5, temperature not specified in the publication | Acinetobacter radioresistens | |
5.45 | - |
4-methylcatechol | wild type enzyme, in 100 mM Tris-HCl pH 8.5, temperature not specified in the publication | Acinetobacter radioresistens | |
5.51 | - |
4-methylcatechol | mutant enzyme A72G, in 100 mM Tris-HCl pH 8.5, temperature not specified in the publication | Acinetobacter radioresistens | |
13.6 | - |
catechol | mutant enzyme A72G, in100 mM Tris-HCl pH 8.5, temperature not specified in the publication | Acinetobacter radioresistens | |
24.2 | - |
catechol | wild type enzyme, in100 mM Tris-HCl pH 8.5, temperature not specified in the publication | Acinetobacter radioresistens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
280 | - |
4-Chlorocatechol | mutant enzyme A72G, in 100 mM Tris-HCl pH 8.5, temperature not specified in the publication | Acinetobacter radioresistens | |
550 | - |
4-methylcatechol | mutant enzyme A72G, in 100 mM Tris-HCl pH 8.5, temperature not specified in the publication | Acinetobacter radioresistens | |
570 | - |
4-methylcatechol | mutant enzyme L69A, in 100 mM Tris-HCl pH 8.5, temperature not specified in the publication | Acinetobacter radioresistens | |
880 | - |
3-methylcatechol | mutant enzyme L69A, in 100 mM Tris-HCl pH 8.5, temperature not specified in the publication | Acinetobacter radioresistens | |
1000 | - |
4-methylcatechol | wild type enzyme, in 100 mM Tris-HCl pH 8.5, temperature not specified in the publication | Acinetobacter radioresistens | |
1460 | - |
catechol | mutant enzyme A72G, in 100 mM Tris-HCl pH 8.5, temperature not specified in the publication | Acinetobacter radioresistens | |
3440 | - |
4-Chlorocatechol | mutant enzyme L69A, in 100 mM Tris-HCl pH 8.5, temperature not specified in the publication | Acinetobacter radioresistens | |
4200 | - |
4-Chlorocatechol | wild type enzyme, in 100 mM Tris-HCl pH 8.5, temperature not specified in the publication | Acinetobacter radioresistens | |
7100 | - |
3-methylcatechol | wild type enzyme, in 100 mM Tris-HCl pH 8.5, temperature not specified in the publication | Acinetobacter radioresistens | |
7800 | - |
catechol | mutant enzyme L69A, in 100 mM Tris-HCl pH 8.5, temperature not specified in the publication | Acinetobacter radioresistens | |
11800 | - |
catechol | wild type enzyme, in 100 mM Tris-HCl pH 8.5, temperature not specified in the publication | Acinetobacter radioresistens |