Literature summary for 1.13.11.1 extracted from

Matera, I.; Ferraroni, M.; Kolomytseva, M.; Golovleva, L.; Scozzafava, A.; Briganti, F.
Catechol 1,2-dioxygenase from the Gram-positive Rhodococcus opacus 1CP: Quantitative structure/activity relationship and the crystal structures of native enzyme and catechols adducts (2010), J. Struct. Biol., 170, 548-564.

Search for more literature for 1.13.11.1

Crystallization (Commentary)

Crystallization (Comment)	Organism
sitting drop vapor diffusion method, using 30% (w/v) PEG400, 0.1 M MES pH 6.5, 0.1 M magnesium chloride	Rhodococcus opacus

Inhibitors

Inhibitors	Comment	Organism	Structure
3,4,5,6-tetrachlorocatechol	competitive inhibitor	Rhodococcus opacus
3,5-dichlorocatechol	competitive inhibitor	Rhodococcus opacus
4,5-dichlorocatechol	competitive inhibitor	Rhodococcus opacus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0007	-	3-chlorocatechol	in 50 mM Tris/HCl, pH 7.2, 25°C	Rhodococcus opacus
0.0015	-	4-Chlorocatechol	in 50 mM Tris/HCl, pH 7.2, 25°C	Rhodococcus opacus
0.003	-	catechol	in 50 mM Tris/HCl, pH 7.2, 25°C	Rhodococcus opacus
0.004	-	4-methylcatechol	in 50 mM Tris/HCl, pH 7.2, 25°C	Rhodococcus opacus
0.0045	-	pyrogallol	in 50 mM Tris/HCl, pH 7.2, 25°C	Rhodococcus opacus
0.0065	-	3-methylcatechol	in 50 mM Tris/HCl, pH 7.2, 25°C	Rhodococcus opacus
0.097	-	Hydroxyquinol	in 50 mM Tris/HCl, pH 7.2, 25°C	Rhodococcus opacus
0.31	-	protocatechuate	in 50 mM Tris/HCl, pH 7.2, 25°C	Rhodococcus opacus
0.7	-	2,3-Dihydroxybenzoic acid	in 50 mM Tris/HCl, pH 7.2, 25°C	Rhodococcus opacus

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Fe3+	contains Fe3+	Rhodococcus opacus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
33000	-	SDS-PAGE	Rhodococcus opacus

Organism

Organism	UniProt	Comment	Textmining
Rhodococcus opacus	P95607	-	-
Rhodococcus opacus 1CP	P95607	-	-

Purification (Commentary)

Purification (Comment)	Organism
Q-Sepharose column chromatography, phenyl-Sepharose column chromatography, and Superdex 200 gel filtration	Rhodococcus opacus

Source Tissue

Source Tissue	Comment	Organism	Textmining
culture condition:benzoate-grown cell	-	Rhodococcus opacus	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2,3-dihydroxybenzoic acid + O2	-	Rhodococcus opacus	?	-	?
3,4,5,6-tetrachlorocatechol + O2	-	Rhodococcus opacus	?	-	?
3,5-dichlorocatechol + O2	-	Rhodococcus opacus	?	-	?
3-chlorocatechol + O2	1.7% activity compared to catechol	Rhodococcus opacus	2-chloro-cis,cis-muconate	-	?
3-chlorocatechol + O2	1.7% activity compared to catechol	Rhodococcus opacus 1CP	2-chloro-cis,cis-muconate	-	?
3-methylcatechol + O2	104% activity compared to catechol	Rhodococcus opacus	(2Z,4Z)-2-methylhexa-2,4-dienedioate	-	?
3-methylcatechol + O2	104% activity compared to catechol	Rhodococcus opacus 1CP	(2Z,4Z)-2-methylhexa-2,4-dienedioate	-	?
4,5-dichlorocatechol + O2	-	Rhodococcus opacus	?	-	?
4-chlorocatechol + O2	2.7% activity compared to catechol	Rhodococcus opacus	(2E,4Z)-3-chlorohexa-2,4-dienedioate	-	?
4-chlorocatechol + O2	2.7% activity compared to catechol	Rhodococcus opacus 1CP	(2E,4Z)-3-chlorohexa-2,4-dienedioate	-	?
4-methylcatechol + O2	71.5% activity compared to catechol	Rhodococcus opacus	4-methyl-cis,cis-muconate	-	?
catechol + O2	100% activity	Rhodococcus opacus	cis,cis-muconate	-	?
catechol + O2	100% activity	Rhodococcus opacus 1CP	cis,cis-muconate	-	?
hydroxyquinol + O2	i.e. 1,2,4-trihydroxy benzene	Rhodococcus opacus	?	-	?
protocatechuate + O2	i.e. 3,4-dihydroxybenzoate	Rhodococcus opacus	?	-	?
pyrogallol + O2	i.e.benzene-1,2,3-triol	Rhodococcus opacus	alpha-hydroxymuconic acid	-	?
pyrogallol + O2	i.e.benzene-1,2,3-triol	Rhodococcus opacus 1CP	alpha-hydroxymuconic acid	-	?

Subunits

Subunits	Comment	Organism
monomer	1 * 33000, SDS-PAGE	Rhodococcus opacus

Synonyms

Synonyms	Comment	Organism
1,2-CTD	-	Rhodococcus opacus

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.04	-	protocatechuate	in 50 mM Tris/HCl, pH 7.2, 25°C	Rhodococcus opacus
0.18	-	3-chlorocatechol	in 50 mM Tris/HCl, pH 7.2, 25°C	Rhodococcus opacus
0.27	-	4-Chlorocatechol	in 50 mM Tris/HCl, pH 7.2, 25°C	Rhodococcus opacus
0.28	-	Hydroxyquinol	in 50 mM Tris/HCl, pH 7.2, 25°C	Rhodococcus opacus
0.28	-	2,3-Dihydroxybenzoic acid	in 50 mM Tris/HCl, pH 7.2, 25°C	Rhodococcus opacus
2.41	-	pyrogallol	in 50 mM Tris/HCl, pH 7.2, 25°C	Rhodococcus opacus
7.32	-	4-methylcatechol	in 50 mM Tris/HCl, pH 7.2, 25°C	Rhodococcus opacus
10.23	-	catechol	in 50 mM Tris/HCl, pH 7.2, 25°C	Rhodococcus opacus
10.62	-	3-methylcatechol	in 50 mM Tris/HCl, pH 7.2, 25°C	Rhodococcus opacus

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.00002	-	4,5-dichlorocatechol	in 50 mM Tris/HCl, pH 7.2, 25°C	Rhodococcus opacus
0.00009	-	3,5-dichlorocatechol	in 50 mM Tris/HCl, pH 7.2, 25°C	Rhodococcus opacus
0.036	-	3,4,5,6-tetrachlorocatechol	in 50 mM Tris/HCl, pH 7.2, 25°C	Rhodococcus opacus

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
0.15	-	protocatechuate	in 50 mM Tris/HCl, pH 7.2, 25°C	Rhodococcus opacus
0.4	-	2,3-Dihydroxybenzoic acid	in 50 mM Tris/HCl, pH 7.2, 25°C	Rhodococcus opacus
2.8	-	Hydroxyquinol	in 50 mM Tris/HCl, pH 7.2, 25°C	Rhodococcus opacus
182	-	4-Chlorocatechol	in 50 mM Tris/HCl, pH 7.2, 25°C	Rhodococcus opacus
255	-	3-chlorocatechol	in 50 mM Tris/HCl, pH 7.2, 25°C	Rhodococcus opacus
535	-	pyrogallol	in 50 mM Tris/HCl, pH 7.2, 25°C	Rhodococcus opacus
1633	-	3-methylcatechol	in 50 mM Tris/HCl, pH 7.2, 25°C	Rhodococcus opacus
1828	-	4-methylcatechol	in 50 mM Tris/HCl, pH 7.2, 25°C	Rhodococcus opacus
3410	-	catechol	in 50 mM Tris/HCl, pH 7.2, 25°C	Rhodococcus opacus

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Release 2023.1 (January 2023)