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Literature summary for 1.13.11.1 extracted from

  • Guzik, U.; Gren, I.; Hupert-Kocurek, K.; Wojcieszynska, D.
    Catechol 1,2-dioxygenase from the new aromatic compounds - degrading Pseudomonas putida strain N6 (2011), Int. Biodeter. Biodegrad., 65, 504-512.
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
gene catA, DNA and amino acid sequence determination and analysis, phylogenetic tree Pseudomonas putida

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
catechol + O2 Pseudomonas putida
-
cis,cis-muconate
-
?
catechol + O2 Pseudomonas putida N6
-
cis,cis-muconate
-
?

Organism

Organism UniProt Comment Textmining
Pseudomonas putida A7LBQ4 strain isolated from the activated sludge samples of a sewage treatment plant at Bentwood Furniture Factory Jasienica, Poland, gene catA
-
Pseudomonas putida N6 A7LBQ4 strain isolated from the activated sludge samples of a sewage treatment plant at Bentwood Furniture Factory Jasienica, Poland, gene catA
-

Source Tissue

Source Tissue Comment Organism Textmining
additional information when cells of strain N6 grow on protocatechuate or 4-hydroxybenzoic acid mainly protocatechuate 3,4-dioxygenases induced. The activity of catechol 1,2-dioxygenase is rather small. The cells grown on benzoic acid, catechol or phenol show high activity of only catechol 1,2-dioxygenase Pseudomonas putida
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
672
-
purified enzyme, pH 7.4, 35°C Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3,4-dihydroxybenzoic acid + O2
-
Pseudomonas putida ?
-
?
3,4-dihydroxybenzoic acid + O2
-
Pseudomonas putida N6 ?
-
?
4-hydroxybenzoic acid + O2
-
Pseudomonas putida ?
-
?
4-hydroxybenzoic acid + O2
-
Pseudomonas putida N6 ?
-
?
benzoic acid + O2
-
Pseudomonas putida ?
-
?
catechol + O2
-
Pseudomonas putida cis,cis-muconate
-
?
catechol + O2
-
Pseudomonas putida N6 cis,cis-muconate
-
?
additional information no activity with vanillic acid Pseudomonas putida ?
-
?
additional information no activity with vanillic acid Pseudomonas putida N6 ?
-
?
phenol + O2 best substrate Pseudomonas putida ?
-
?
phenol + O2 best substrate Pseudomonas putida N6 ?
-
?

Subunits

Subunits Comment Organism
More three-dimensional structure prediction, overview Pseudomonas putida

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
-
Pseudomonas putida

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
6 60 activity range, profile overview Pseudomonas putida

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
-
Pseudomonas putida

pH Range

pH Minimum pH Maximum Comment Organism
5 9 activity range, profile overview Pseudomonas putida