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Literature summary for 1.13.11.12 extracted from

  • Park, J.Y.; Kim, C.H.; Choi, Y.; Park, K.M.; Chang, P.S.
    Catalytic characterization of heterodimeric linoleate 13S-lipoxygenase from black soybean (Glycine max (L.) Merr.) (2020), Enzyme Microb. Technol., 139, 109595 .
    View publication on PubMed
Search for more literature for 1.13.11.12

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ about 70% inhibition at 20 mM Glycine max
Hg2+ about 80% inhibition at 20 mM Glycine max
Zn2+ complete inhibition at 20 mM Glycine max

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.636
-
 linoleic acid at pH 9.5 and 40°C Glycine max

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ 129.79% activity at 20 mM Glycine max
additional information Mg2+ and Ca2+ have no effect on the enzyme activity Glycine max

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
101000
-
 gel filtration Glycine max

Organism

Organism UniProt Comment Textmining
Glycine max
-
 black soybean
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, HiTrap Q column chromatography, DEAE Sepharose column chromatography, and Sephacryl S-100 gel filtration Glycine max

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.04
-
 crude enzyme, at pH 9.5 and 40°C Glycine max
3
-
 after 85fold purification, at pH 9.5 and 40°C Glycine max

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-linolenic acid + O2 minor substrate Glycine max ?
-
 ?
linoleic acid + O2
-
 Glycine max 13(S)-hydroperoxy-9,11-octadecadienoic acid
-
 ?
additional information no activity with oleic acid Glycine max ?
-
-

Subunits

Subunits Comment Organism
heterodimer 1 * 55000 + 1 * 46000, SDS-PAGE Glycine max

Synonyms

Synonyms Comment Organism
13S-lipoxygenase
-
 Glycine max
LOX
-
 Glycine max

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Glycine max

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
15 55 the enzyme has 51.51% activity at 15°C and 63.97% activity at 55°C Glycine max

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40 70 the enzyme shows no loss of catalytic activity at temperatures up to 40°C after 15 min incubation. When the temperature increases above 40 °C, the enzyme dramatically loses its catalytic activity by 56% at 60°C and is fully deactivated at 70°C Glycine max

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
12.28
-
 linoleic acid at pH 9.5 and 40°C Glycine max

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.5
-
-
 Glycine max

pH Range

pH Minimum pH Maximum Comment Organism
8 10 the enzyme has about 60% activity at pH 8.0 and about 70% activity at pH10.0 Glycine max

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 10 the enzyme is highly stable at pH 6.0-10.0, retaining over 80% of its original catalytic activity after 24 h Glycine max

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
19.3
-
 linoleic acid at pH 9.5 and 40°C Glycine max