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Literature summary for 1.13.11.15 extracted from

  • Jamaluddin, M.P.
    Purification and properties of homoprotocatechuate 2,3-dioxygenase from Bacillus stearothermophilus (1977), J. Bacteriol., 129, 690-697.
    View publication on PubMedView publication on EuropePMC

Inhibitors

Inhibitors Comment Organism Structure
dithiothreitol 0.1 mM, 13% inhibition Geobacillus stearothermophilus
Ferrous ammonium sulfate 0.1 mM, 32% inhibition Geobacillus stearothermophilus
p-hydroxymercuribenzoate 0.1 mM, 13% inhibition Geobacillus stearothermophilus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0034
-
3,4-Dihydroxyphenylacetate 32°C Geobacillus stearothermophilus
0.0037
-
3,4-Dihydroxyphenylpropionic acid 32°C Geobacillus stearothermophilus
0.004
-
3,4-Dihydroxyphenylacetate 28°C Geobacillus stearothermophilus
0.0047
-
3,4-Dihydroxyphenylacetate 21°C Geobacillus stearothermophilus
0.0058
-
3,4-Dihydroxyphenylacetate 38°C Geobacillus stearothermophilus
0.0074
-
3,4-Dihydroxyphenylacetate 47°C Geobacillus stearothermophilus
0.035
-
DL-3,4-dihydroxymandelic acid 32°C Geobacillus stearothermophilus
0.45
-
3,4-dihydroxybenzoic acid
-
Geobacillus stearothermophilus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
106000
-
gel filtration Geobacillus stearothermophilus

Organism

Organism UniProt Comment Textmining
Geobacillus stearothermophilus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Geobacillus stearothermophilus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.25
-
-
Geobacillus stearothermophilus

Storage Stability

Storage Stability Organism
room temperature, little loss of activity after 3 months Geobacillus stearothermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3,4-dihydroxybenzoic acid + O2 0.58% of the activity with 3,4-dihydroxyphenylacetate Geobacillus stearothermophilus 2-hydroxy-5-carboxymuconate semialdehyde
-
?
3,4-dihydroxymandelate + O2 83% of the activity with 3,4-dihydroxyphenylacetate Geobacillus stearothermophilus ?
-
?
3,4-dihydroxyphenylacetate + O2
-
Geobacillus stearothermophilus 2-hydroxy-5-carboxymethylmuconate semialdehyde
-
?
3,4-dihydroxyphenylpropionate + O2 58% of the activity with 3,4-dihydroxyphenylacetate Geobacillus stearothermophilus 2-hydroxy-5-carboxyethylmuconate semialdehyde
-
?

Subunits

Subunits Comment Organism
? x * 33000-35000 Geobacillus stearothermophilus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
57
-
at pH 8.2 Geobacillus stearothermophilus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 64 40°C: about 40% of maximal activity, 64°C: about 75% of maximal activity Geobacillus stearothermophilus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
63
-
2 h, 5% loss of activity Geobacillus stearothermophilus
88
-
10 min, 50% loss of activity Geobacillus stearothermophilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.4 8.7
-
Geobacillus stearothermophilus

pH Range

pH Minimum pH Maximum Comment Organism
7.1 9.3 78% of maximal activity at pH 7.1 and 9.3 Geobacillus stearothermophilus