BRENDA - Enzyme Database show
show all sequences of 1.13.11.16

Catechol dioxygenases from Escherichia coli (MhpB) and Alcaligenes eutrophus (MpcI): sequence analysis and biochemical properties of a third family of extradiol dioxygenases

Spence, E.L.; Kawamukai, M.; Sanvoisin, J.; Braven, H.; Bugg, T.D.; J. Bacteriol. 178, 5249-5256 (1996)

Data extracted from this reference:

General Stability
General Stability
Organism
activity stable in crude extract, inactive apoenzyme stable, can be re-activated by treatment with Fe2+ and ascorbate, irreversible apoenzyme activity loss if stored in completely Fe2+-free buffer, re-activated holoenzyme unstable
Cupriavidus necator
activity stable in crude extract, inactive apoenzyme stable, can be re-activated by treatment with Fe2+ and ascorbate, irreversible apoenzyme activity loss if stored in completely Fe2+-free buffer, re-activated holoenzyme unstable
Escherichia coli
Inhibitors
Inhibitors
Commentary
Organism
Structure
diethyl dicarbonate
-
Cupriavidus necator
diethyl dicarbonate
-
Escherichia coli
DTNB
-
Cupriavidus necator
DTNB
-
Escherichia coli
p-hydroxymercuribenzoate
-
Cupriavidus necator
p-hydroxymercuribenzoate
-
Escherichia coli
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0071
-
3-(2,3-dihydroxyphenyl)propionate
-
Cupriavidus necator
0.014
-
2,3-dihydroxycinnamic acid
-
Cupriavidus necator
0.036
-
2,3-dihydroxycinnamic acid
-
Escherichia coli
0.037
-
methyl-2,3-dihydroxyphenylpropionate
-
Escherichia coli
0.059
-
3-ethylcatechol
-
Cupriavidus necator
0.094
-
3-phenethylcatechol
-
Escherichia coli
0.13
-
3-methylcatechol
-
Cupriavidus necator
0.154
-
3-propylcatechol
-
Escherichia coli
0.185
-
3-ethylcatechol
-
Escherichia coli
0.3
-
2,3-dihydroxyphenoxyacetic acid
-
Escherichia coli
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3-(2,3-dihydroxyphenyl)propionate + O2
Cupriavidus necator
-
2-hydroxy-6-oxonona-2,4-diene-1,9-dioate
-
-
?
3-(2,3-dihydroxyphenyl)propionate + O2
Escherichia coli
-
2-hydroxy-6-oxonona-2,4-diene-1,9-dioate
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Cupriavidus necator
-
-
-
Escherichia coli
P0ABR9
gene mhpB
-
Purification (Commentary)
Commentary
Organism
-
Cupriavidus necator
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.3
-
2,3-dihydroxyphenylproponic acid
Cupriavidus necator
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2,3-dihydroxycinnamic acid + O2
-
395596
Cupriavidus necator
2-hydroxy-6-oxonona-2,4,7-trienoate
-
-
-
?
2,3-dihydroxycinnamic acid + O2
-
395596
Escherichia coli
2-hydroxy-6-oxonona-2,4,7-trienoate
-
-
-
?
2,3-dihydroxyphenoxyacetic acid + O2
-
395596
Escherichia coli
2-hydroxyhexa-2,4-dienoic acid-6-carboxymethylester
-
-
-
?
2,3-dihydroxyphenylpropionic acid + O2
-
395596
Cupriavidus necator
2-hydroxy-6-oxonona-dienoate
-
-
-
?
2,3-dihydroxyphenylpropionic acid + O2
-
395596
Escherichia coli
2-hydroxy-6-oxonona-dienoate
-
-
-
?
3-(2,3-dihydroxyphenyl)propionate + O2
-
395596
Cupriavidus necator
2-hydroxy-6-oxonona-2,4-diene-1,9-dioate
-
-
-
?
3-(2,3-dihydroxyphenyl)propionate + O2
-
395596
Escherichia coli
2-hydroxy-6-oxonona-2,4-diene-1,9-dioate
-
-
-
?
3-ethylcatechol + O2
-
395596
Cupriavidus necator
2-hydroxy-6-oxoocta-2,4-dienoate
-
-
-
?
3-ethylcatechol + O2
-
395596
Escherichia coli
2-hydroxy-6-oxoocta-2,4-dienoate
-
-
-
?
3-methylcatechol + O2
-
395596
Cupriavidus necator
2-hydroxy-6-oxohepta-2,4-dienoate
-
-
-
?
3-methylcatechol + O2
meta-ring cleavage
395596
Escherichia coli
2-hydroxy-6-oxohepta-2,4-dienoate
-
-
-
?
3-phenethylcatechol + O2
-
395596
Escherichia coli
2-hydroxy-6-oxo-8-phenylocta-2,4-dienoate
-
-
-
?
3-propylcatechol + O2
-
395596
Escherichia coli
2-hydroxy-6-oxonona-2,4-dienoate
-
-
-
?
methyl-(2,3-dihydroxyphenyl)propionate + O2
-
395596
Escherichia coli
2-hydroxy-6-oxonona-2,4-dienoic acid-9-methylester
-
-
-
?
General Stability (protein specific)
General Stability
Organism
activity stable in crude extract, inactive apoenzyme stable, can be re-activated by treatment with Fe2+ and ascorbate, irreversible apoenzyme activity loss if stored in completely Fe2+-free buffer, re-activated holoenzyme unstable
Cupriavidus necator
activity stable in crude extract, inactive apoenzyme stable, can be re-activated by treatment with Fe2+ and ascorbate, irreversible apoenzyme activity loss if stored in completely Fe2+-free buffer, re-activated holoenzyme unstable
Escherichia coli
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
diethyl dicarbonate
-
Cupriavidus necator
diethyl dicarbonate
-
Escherichia coli
DTNB
-
Cupriavidus necator
DTNB
-
Escherichia coli
p-hydroxymercuribenzoate
-
Cupriavidus necator
p-hydroxymercuribenzoate
-
Escherichia coli
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0071
-
3-(2,3-dihydroxyphenyl)propionate
-
Cupriavidus necator
0.014
-
2,3-dihydroxycinnamic acid
-
Cupriavidus necator
0.036
-
2,3-dihydroxycinnamic acid
-
Escherichia coli
0.037
-
methyl-2,3-dihydroxyphenylpropionate
-
Escherichia coli
0.059
-
3-ethylcatechol
-
Cupriavidus necator
0.094
-
3-phenethylcatechol
-
Escherichia coli
0.13
-
3-methylcatechol
-
Cupriavidus necator
0.154
-
3-propylcatechol
-
Escherichia coli
0.185
-
3-ethylcatechol
-
Escherichia coli
0.3
-
2,3-dihydroxyphenoxyacetic acid
-
Escherichia coli
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3-(2,3-dihydroxyphenyl)propionate + O2
Cupriavidus necator
-
2-hydroxy-6-oxonona-2,4-diene-1,9-dioate
-
-
?
3-(2,3-dihydroxyphenyl)propionate + O2
Escherichia coli
-
2-hydroxy-6-oxonona-2,4-diene-1,9-dioate
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Cupriavidus necator
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.3
-
2,3-dihydroxyphenylproponic acid
Cupriavidus necator
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2,3-dihydroxycinnamic acid + O2
-
395596
Cupriavidus necator
2-hydroxy-6-oxonona-2,4,7-trienoate
-
-
-
?
2,3-dihydroxycinnamic acid + O2
-
395596
Escherichia coli
2-hydroxy-6-oxonona-2,4,7-trienoate
-
-
-
?
2,3-dihydroxyphenoxyacetic acid + O2
-
395596
Escherichia coli
2-hydroxyhexa-2,4-dienoic acid-6-carboxymethylester
-
-
-
?
2,3-dihydroxyphenylpropionic acid + O2
-
395596
Cupriavidus necator
2-hydroxy-6-oxonona-dienoate
-
-
-
?
2,3-dihydroxyphenylpropionic acid + O2
-
395596
Escherichia coli
2-hydroxy-6-oxonona-dienoate
-
-
-
?
3-(2,3-dihydroxyphenyl)propionate + O2
-
395596
Cupriavidus necator
2-hydroxy-6-oxonona-2,4-diene-1,9-dioate
-
-
-
?
3-(2,3-dihydroxyphenyl)propionate + O2
-
395596
Escherichia coli
2-hydroxy-6-oxonona-2,4-diene-1,9-dioate
-
-
-
?
3-ethylcatechol + O2
-
395596
Cupriavidus necator
2-hydroxy-6-oxoocta-2,4-dienoate
-
-
-
?
3-ethylcatechol + O2
-
395596
Escherichia coli
2-hydroxy-6-oxoocta-2,4-dienoate
-
-
-
?
3-methylcatechol + O2
-
395596
Cupriavidus necator
2-hydroxy-6-oxohepta-2,4-dienoate
-
-
-
?
3-methylcatechol + O2
meta-ring cleavage
395596
Escherichia coli
2-hydroxy-6-oxohepta-2,4-dienoate
-
-
-
?
3-phenethylcatechol + O2
-
395596
Escherichia coli
2-hydroxy-6-oxo-8-phenylocta-2,4-dienoate
-
-
-
?
3-propylcatechol + O2
-
395596
Escherichia coli
2-hydroxy-6-oxonona-2,4-dienoate
-
-
-
?
methyl-(2,3-dihydroxyphenyl)propionate + O2
-
395596
Escherichia coli
2-hydroxy-6-oxonona-2,4-dienoic acid-9-methylester
-
-
-
?
Other publictions for EC 1.13.11.16
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
742063
Sainsbury
Chemical intervention in bact ...
Rhodococcus jostii
Bioorg. Chem.
60
102-109
2015
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2
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4
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1
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1
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1
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-
1
2
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-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
395596
Spence
Catechol dioxygenases from Esc ...
Cupriavidus necator, Escherichia coli
J. Bacteriol.
178
5249-5256
1996
-
-
-
-
-
2
6
10
-
-
-
2
-
6
-
-
1
-
-
-
1
-
14
-
-
-
-
-
-
-
-
-
-
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-
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-
2
-
6
-
10
-
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2
-
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-
1
-
-
1
-
14
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
395595
Bugg
Overproduction, purification a ...
Escherichia coli
Biochim. Biophys. Acta
1202
258-264
1993
-
-
-
-
-
1
2
3
-
1
2
1
-
4
-
-
1
-
-
-
1
-
4
1
-
-
-
3
1
-
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-
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-
1
-
2
-
3
-
1
2
1
-
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-
1
-
-
1
-
4
1
-
-
-
3
1
-
-
-
-
-
-
-
-
-
395593
Geary
The time sequence of interacti ...
Achromobacter sp.
Biochim. Biophys. Acta
167
459-461
1968
-
-
-
-
-
-
-
-
-
1
1
1
-
1
-
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1
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1
1
1
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1
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395594
Dagley
The metabolism of beta-phenylp ...
Achromobacter sp.
Biochem. J.
97
643-650
1965
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1
-
1
-
1
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1
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1
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1
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1
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1
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1
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