BRENDA - Enzyme Database show
show all sequences of 1.13.11.16

Chemical intervention in bacterial lignin degradation pathways development of selective inhibitors for intradiol and extradiol catechol dioxygenases

Sainsbury, P.D.; Mineyeva, Y.; Mycroft, Z.; Bugg, T.D.; Bioorg. Chem. 60, 102-109 (2015)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
2-(3,4-dihydroxyphenyl)-N-[(4-fluorophenyl)methyl]-N-hydroxyacetamide
over 90% inhibition at 0.25 mM
Rhodococcus jostii
N-benzyl-3-(3,4-dimethoxyphenyl)-N-hydroxypropanamide
25% inhibition at 0.25 mM
Rhodococcus jostii
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Rhodococcus jostii
-
-
-
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Rhodococcus jostii
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
assay at
Rhodococcus jostii
IC50 Value
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
0.11
-
pH 7.5, 37°C
Rhodococcus jostii
2-(3,4-dihydroxyphenyl)-N-[(4-fluorophenyl)methyl]-N-hydroxyacetamide
IC50 Value (protein specific)
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
0.11
-
pH 7.5, 37°C
Rhodococcus jostii
2-(3,4-dihydroxyphenyl)-N-[(4-fluorophenyl)methyl]-N-hydroxyacetamide
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
2-(3,4-dihydroxyphenyl)-N-[(4-fluorophenyl)methyl]-N-hydroxyacetamide
over 90% inhibition at 0.25 mM
Rhodococcus jostii
N-benzyl-3-(3,4-dimethoxyphenyl)-N-hydroxypropanamide
25% inhibition at 0.25 mM
Rhodococcus jostii
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Rhodococcus jostii
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
assay at
Rhodococcus jostii
Other publictions for EC 1.13.11.16
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
742063
Sainsbury
Chemical intervention in bact ...
Rhodococcus jostii
Bioorg. Chem.
60
102-109
2015
-
-
-
-
-
-
2
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
1
2
-
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-
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-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
395596
Spence
Catechol dioxygenases from Esc ...
Cupriavidus necator, Escherichia coli
J. Bacteriol.
178
5249-5256
1996
-
-
-
-
-
2
6
10
-
-
-
2
-
6
-
-
1
-
-
-
1
-
14
-
-
-
-
-
-
-
-
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-
-
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-
-
2
-
6
-
10
-
-
-
2
-
-
-
1
-
-
1
-
14
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
395595
Bugg
Overproduction, purification a ...
Escherichia coli
Biochim. Biophys. Acta
1202
258-264
1993
-
-
-
-
-
1
2
3
-
1
2
1
-
4
-
-
1
-
-
-
1
-
4
1
-
-
-
3
1
-
-
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-
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-
1
-
2
-
3
-
1
2
1
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-
-
1
-
-
1
-
4
1
-
-
-
3
1
-
-
-
-
-
-
-
-
-
395593
Geary
The time sequence of interacti ...
Achromobacter sp.
Biochim. Biophys. Acta
167
459-461
1968
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-
-
-
-
-
-
-
-
1
1
1
-
1
-
-
-
-
-
-
-
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1
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-
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1
1
1
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1
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395594
Dagley
The metabolism of beta-phenylp ...
Achromobacter sp.
Biochem. J.
97
643-650
1965
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-
-
-
-
-
-
-
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1
-
1
-
1
-
-
1
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1
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-
1
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1
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1
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1
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